肝细胞癌患者肝脏组织中的维生素K含量
Vitamin K contents in liver tissue of hepatocellular carcinoma patients.
作者信息
Miyakawa T, Kajiwara Y, Shirahata A, Okamoto K, Itoh H, Ohsato K
机构信息
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, Japan.
出版信息
Jpn J Cancer Res. 2000 Jan;91(1):68-74. doi: 10.1111/j.1349-7006.2000.tb00861.x.
Serum protein induced in vitamin K absence-II (PIVKA-II) is used as a tumor marker because it increases at a notably higher rate in patients with hepatocellular carcinoma. To clarify the mechanism causing the elevation of serum PIVKA-II, we measured the contents of vitamins K1 (phylloquinone, PK) and K2 (menaquinone, MK) (MK-4, MK-5, MK-6, MK-7, MK-8, MK-9, MK-10) in liver tissue resected from 21 hepatic cancer patients (12 patients with hepatocellular carcinoma and 9 patients with metastatic hepatic cancer), using HPLC combined with coulometric reduction and fluorometric detection. In the cancerous tissue of hepatocellular carcinoma patients, PK, MK-7, MK-8, and MK-10 were significantly lower than that found in the noncancerous tissue. Furthermore, MK-6, MK-7, MK-8, and MK-10 in the cancerous tissue of hepatocellular carcinoma patients were significantly lower than that in the cancerous tissue of metastatic hepatic cancer patients. These data suggested that one of the mechanisms of the elevation of serum PIVKA-II levels in hepatocellular carcinoma patients is a vitamin K deficiency in the local cancerous tissue.
维生素K缺乏诱导蛋白-II（PIVKA-II）用作肿瘤标志物，因为其在肝细胞癌患者中升高速率显著更高。为阐明血清PIVKA-II升高的机制，我们采用高效液相色谱结合库仑还原和荧光检测法，测定了21例肝癌患者（12例肝细胞癌患者和9例转移性肝癌患者）切除的肝组织中维生素K1（叶绿醌，PK）和维生素K2（甲萘醌，MK）（MK-4、MK-5、MK-6、MK-7、MK-8、MK-9、MK-10）的含量。在肝细胞癌患者的癌组织中，PK、MK-7、MK-8和MK-10显著低于非癌组织。此外，肝细胞癌患者癌组织中的MK-6、MK-7、MK-8和MK-10显著低于转移性肝癌患者癌组织中的含量。这些数据表明，肝细胞癌患者血清PIVKA-II水平升高的机制之一是局部癌组织中维生素K缺乏。
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