cagE是一种与幽门螺杆菌诱发儿童十二指肠溃疡相关的毒力因子。
cagE is a virulence factor associated with Helicobacter pylori-induced duodenal ulceration in children.
作者信息
Day A S, Jones N L, Lynett J T, Jennings H A, Fallone C A, Beech R, Sherman P M
机构信息
Division of Gastroenterology and Nutrition, Research Institute, Hospital for Sick Children, University of Toronto, Canada.
出版信息
J Infect Dis. 2000 Apr;181(4):1370-5. doi: 10.1086/315394. Epub 2000 Apr 13.
This study was undertaken to determine whether infection with Helicobacter pylori strains that contain the cagE gene was associated with duodenal ulceration in children. The presence of flaA, cagA, and cagE genes was determined by polymerase chain reaction in H. pylori previously cultured from 29 children. Twelve (92%) of 13 children with duodenal ulcers were infected with cagE-positive isolates, compared with only 5 (31%) of 16 with gastritis alone (P<.01). Infection of gastric cells in tissue culture by cagE-positive H. pylori resulted in greater increments in interleukin-8 levels compared with cagE-negative strains (2.3+/-0.1 vs. 1.3+/-0.2 ng/mL in AGS cells [P<.005]; 1.5+/-0.3 vs. 0.5+/-0.2 ng/mL in KATO-III cells [P<.05]). H. pylori-containing cagE was associated with the presence of duodenal ulceration in children. Enhanced chemokine production after infection with cagE-positive H. pylori could affect disease outcome.
本研究旨在确定感染携带cagE基因的幽门螺杆菌菌株是否与儿童十二指肠溃疡有关。通过聚合酶链反应测定了先前从29名儿童中培养出的幽门螺杆菌中flaA、cagA和cagE基因的存在情况。13名十二指肠溃疡儿童中有12名（92%）感染了cagE阳性菌株，而16名单纯胃炎儿童中只有5名（31%）感染了该菌株（P<0.01）。与cagE阴性菌株相比，cagE阳性幽门螺杆菌在组织培养中感染胃细胞后导致白细胞介素-8水平有更大幅度的升高（AGS细胞中分别为2.3±0.1 vs. 1.3±0.2 ng/mL [P<0.005]；KATO-III细胞中分别为1.5±0.3 vs. 0.5±0.2 ng/mL [P<0.05]）。携带cagE的幽门螺杆菌与儿童十二指肠溃疡的存在有关。cagE阳性幽门螺杆菌感染后趋化因子产生增强可能影响疾病结局。
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