Patients Diagnostic Lab, Isotope Application Division, Pakistan Institute of Nuclear Science and Technology (PINSTECH), Islamabad 44000, Pakistan.
Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Molecules. 2022 Aug 24;27(17):5410. doi: 10.3390/molecules27175410.
(1) Background: Gastric cancer (GC) is the fourth leading cause of cancer-related deaths worldwide. infection is a major risk factor, but other microbial species may also be involved. In the context of an earlier proteomics study of serum and biopsies of patients with gastroduodenal diseases, we explored here a simplified microbiome in these biopsies (, , , , ) on the protein level. (2) Methods: A cohort of 75 patients was divided into groups with respect to the findings of the normal gastric mucosa (NGM) and gastroduodenal disorders such as gastritis, ulcer, and gastric cancer (GC). The infection status was determined. The protein expression analysis of the biopsy samples was carried out using high-definition mass spectrometry of the tryptic digest (label-free data-independent quantification and statistical analysis). (3) Results: The total of 304 bacterial protein matches were detected based on two or more peptide hits. Significantly regulated microbial proteins like virulence factor type IV secretion system protein CagE from were found with more abundance in gastritis than in GC or NGM. This finding could reflect the increased microbial involvement in mucosa inflammation in line with current hypotheses. Abundant proteins across species were heat shock proteins and elongation factors. (4) Conclusions: Next to the bulk of human proteins, a number of species-specific bacterial proteins were detected in stomach biopsies of patients with gastroduodenal diseases, some of which, like those expressed by the pathogenicity island, may provide gateways to disease prevention without antibacterial intervention in order to reduce antibiotic resistance.
(1) 背景：胃癌（GC）是全球第四大癌症相关死亡原因。 感染是一个主要的危险因素，但其他微生物物种也可能参与其中。在对胃十二指肠疾病患者的血清和活检进行早期蛋白质组学研究的背景下，我们在此探索了这些活检标本（血清和活检）中的简化微生物组（血清和活检）在蛋白质水平上。(2) 方法：将 75 名患者分为正常胃黏膜（NGM）和胃十二指肠疾病（如胃炎、溃疡和胃癌（GC））的组。确定了 的感染状态。使用胰蛋白酶消化物的高清晰度质谱（无标记数据独立定量和统计分析）对活检样本进行蛋白质表达分析。(3) 结果：基于两个或更多肽的匹配，共检测到 304 种细菌蛋白。在胃炎中比在 GC 或 NGM 中更丰富地发现了调节微生物蛋白，如来自 的毒力因子 IV 型分泌系统蛋白 CagE。这一发现可能反映了微生物在粘膜炎症中的参与增加，符合当前的假说。跨物种丰富的蛋白质是热休克蛋白和延伸因子。(4) 结论：除了大量的人类蛋白质外，在胃十二指肠疾病患者的胃活检标本中还检测到了一些物种特异性的细菌蛋白质，其中一些，如表达致病性岛的蛋白质，可能为疾病预防提供途径，而无需抗菌干预，以减少抗生素耐药性。
