McGinnis M R, Nordoff N G, Ryder N S, Nunn G B
Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
Antimicrob Agents Chemother. 2000 May;44(5):1407-8. doi: 10.1128/AAC.44.5.1407-1408.2000.
We evaluated terbinafine and itraconazole against 30 isolates of Penicillium marneffei using a modification of the National Committee for Clinical Laboratory Standards broth macrodilution MIC testing protocol for yeasts. The minimal fungicidal concentration (MFC) was determined by plating 100 microl from each MIC drug dilution having no growth onto Sabouraud glucose agar incubated at 30 degrees C. The MFC was the dilution at which growth was absent at 72 h of incubation. The MICs, in micrograms per milliliter, were as follows: terbinafine, 0.03 to 1.0 (geometric mean titer, 0.09); itraconazole, 0.03 to 0.5 (geometric mean titer, 0.04). The MFCs, in micrograms per milliliter, were as follows: terbinafine, 0.03 to 8 (geometric mean titer, 2.60); itraconazole, 0.03 to 8 (geometric mean titer, 2. 45). Primary fungicidal activity (MFC within 2 dilutions of MIC) was observed with terbinafine in eight isolates and with itraconazole in four isolates. The data indicate that terbinafine is active against P. marneffei in vitro and may have a previously unrealized role in the management of infections caused by this fungus.
我们采用针对酵母菌的美国国家临床实验室标准委员会肉汤宏稀释法 MIC 检测方案的改良方法，评估了特比萘芬和伊曲康唑对 30 株马尔尼菲青霉菌株的抗菌活性。通过将每份无生长的 MIC 药物稀释液中的 100 微升接种到 30℃培养的沙氏葡萄糖琼脂平板上，来确定最低杀菌浓度（MFC）。MFC 是在培养 72 小时后无生长的稀释度。MIC 以微克/毫升计，结果如下：特比萘芬，0.03 至 1.0（几何平均滴度，0.09）；伊曲康唑，0.03 至 0.5（几何平均滴度，0.04）。MFC 以微克/毫升计，结果如下：特比萘芬，0.03 至 8（几何平均滴度，2.60）；伊曲康唑，0.03 至 8（几何平均滴度，2.45）。在 8 株菌株中观察到特比萘芬具有主要杀菌活性（MFC 在 MIC 的 2 个稀释度范围内），在 4 株菌株中观察到伊曲康唑具有主要杀菌活性。数据表明，特比萘芬在体外对马尔尼菲青霉具有活性，在该真菌引起的感染治疗中可能具有此前未被认识到的作用。
