Parsons R, Yue V, Tong X, Cardot P, Bernard A, Andreux J P, Caldwell K
Diagnostics Division, Abbott Laboratories, Abbott Park, IL, USA.
J Chromatogr B Biomed Appl. 1996 Nov 15;686(2):177-87. doi: 10.1016/s0378-4347(96)00186-7.
An extensive multi-laboratory study was conducted to compare three different field-flow fractionation (FFF) systems for use in the analysis of human erythrocytes. The object of this study was to determine the relationship between the FFF elution properties for each system and the traditional hematological blood cell parameters. One centrifugal system (Utah) and two gravitational systems (Paris and Abbott) were compared. In order to analyze erythrocyte populations with a broad range of hematological indices, blood samples were collected from individuals heterozygous for sickle cell anemia (A/S) and also from normal controls (A/A), and these were analyzed at each site. Identical samples were analyzed by the Abbott and Utah sites. With all three systems, blood samples from each category produced narrow, overlapping distributions of FFF retention ratios, with the Abbott and Utah systems showing slight elevations in the mean retention ratios for the sickle cell samples. Blood cell elution peak characteristics were compared with standard hematological parameters for each of the FFF systems, and negative correlations were consistently found between mean corpuscular volume (MCV) and retention ratios. Positive correlations were found between red cell distribution width (RDW) and retention ratios. Elevated FFF retention ratios were frequently found with blood samples having abnormal hematological profiles. These results demonstrate that the three differently configured systems all produce similar analysis profiles for erythrocytes from the classes studied here. The relationships between FFF parameters and hematological indices were consistent for all systems.
进行了一项广泛的多实验室研究，以比较三种不同的场流分级（FFF）系统在人类红细胞分析中的应用。本研究的目的是确定每个系统的FFF洗脱特性与传统血液学血细胞参数之间的关系。比较了一种离心系统（犹他州）和两种重力系统（巴黎和雅培）。为了分析具有广泛血液学指标的红细胞群体，从镰状细胞贫血杂合子个体（A/S）以及正常对照（A/A）中采集血样，并在每个站点进行分析。相同的样本由雅培和犹他州的站点进行分析。使用所有三种系统时，每类血样产生的FFF保留率分布狭窄且重叠，雅培和犹他州系统显示镰状细胞样本的平均保留率略有升高。将血细胞洗脱峰特征与每个FFF系统的标准血液学参数进行比较，始终发现平均红细胞体积（MCV）与保留率之间呈负相关。发现红细胞分布宽度（RDW）与保留率之间呈正相关。血液学特征异常的血样经常出现FFF保留率升高的情况。这些结果表明，这里研究的三种不同配置的系统对红细胞都产生了相似的分析图谱。所有系统的FFF参数与血液学指标之间的关系都是一致的。
