Menssen H D, Bertelmann E, Bartelt S, Schmidt R A, Pecher G, Schramm K, Thiel E
Medizinische Klinik III, Hämatologie, Onkologie und Transfusionsmedizin, Benjamin Franklin Klinik der Freien Universität, Berlin, Germany.
J Cancer Res Clin Oncol. 2000 Apr;126(4):226-32. doi: 10.1007/s004320050037.
The Wilms' tumor gene (WT1) encodes a transcriptional regulator involved in growth and differentiation of various tissue types. A continuous over-expression of WT1 was found in leukemic blasts, thus suggesting an oncogenic function. Solid cancer entities have also been described as expressing WT1. We systematically analyzed WT1 expression in small-cell and non-small-cell lung cancer, colon cancer and glioblastoma patients and in the respective tumor cell lines. Using reverse transcription/polymerase chain reaction, we found WT1 expression in glioblastoma (5 of 8), lung (5 of 11), and colon cancer (5 of 15) cell lines. While WT1 was expressed in only 1 of 12 lung cancer and 1 of 5 glioblastoma specimens, it was not detected in colon cancer or macroscopically tumor-free colon and lung tissue. In addition, HT29 colon cancer cells showed a loss of WT1 expression when grown to confluence or induced to differentiate by sodium butyrate. From this evidence, testing for WT1 expression is not clinically relevant for colon cancer, lung cancer, or glioblastoma patients. WT1 expression in cancer cell lines can probably be attributed to optimized in vitro growth conditions.
威尔姆斯瘤基因（WT1）编码一种参与多种组织类型生长和分化的转录调节因子。在白血病原始细胞中发现WT1持续过度表达，因此提示其具有致癌功能。实体癌也被描述为表达WT1。我们系统分析了WT1在小细胞肺癌、非小细胞肺癌、结肠癌和胶质母细胞瘤患者以及相应肿瘤细胞系中的表达情况。通过逆转录/聚合酶链反应，我们在胶质母细胞瘤（8例中的5例）、肺癌（11例中的5例）和结肠癌细胞系（15例中的5例）中发现了WT1表达。虽然WT1仅在12例肺癌标本中的1例以及5例胶质母细胞瘤标本中的1例中表达，但在结肠癌或肉眼无肿瘤的结肠和肺组织中未检测到。此外，HT29结肠癌细胞在汇合生长或用丁酸钠诱导分化时显示WT1表达缺失。基于这些证据，检测WT1表达对结肠癌、肺癌或胶质母细胞瘤患者并无临床意义。癌细胞系中WT1的表达可能归因于优化的体外生长条件。
