Spielmeyer W, Moullet O, Laroche A, Lagudah E S
CSIRO Plant Industry, Canberra ACT 2601, Australia.
Genetics. 2000 May;155(1):361-7. doi: 10.1093/genetics/155.1.361.
A detailed RFLP map was constructed of the distal end of the short arm of chromosome 1D of Aegilops tauschii, the diploid D-genome donor species of hexaploid wheat. Ae. tauschii was used to overcome some of the limitations commonly associated with molecular studies of wheat such as low levels of DNA polymorphism. Detection of multiple loci by most RFLP probes suggests that gene duplication events have occurred throughout this chromosomal region. Large DNA fragments isolated from a BAC library of Ae. tauschii were used to determine the relationship between physical and genetic distance at seed storage protein loci located at the distal end of chromosome 1DS. Highly recombinogenic regions were identified where the ratio of physical to genetic distance was estimated to be <20 kb/cM. These results are discussed in relation to the genome-wide estimate of the relationship between physical and genetic distance.
构建了节节麦（Aegilops tauschii）1D染色体短臂远端的详细限制性片段长度多态性（RFLP）图谱，节节麦是六倍体小麦的二倍体D基因组供体物种。利用节节麦克服了一些与小麦分子研究通常相关的局限性，如DNA多态性水平低。大多数RFLP探针检测到多个位点，表明在整个该染色体区域发生了基因重复事件。从节节麦BAC文库中分离的大DNA片段用于确定位于1DS染色体远端的种子贮藏蛋白基因座处物理距离与遗传距离之间的关系。鉴定出了高重组区域，其中物理距离与遗传距离的比率估计小于20 kb/cM。结合全基因组物理距离与遗传距离关系的估计对这些结果进行了讨论。
