流感嗜血杆菌β-内酰胺酶的碘量法检测：氨苄西林耐药性的快速初步检测
Iodometric detection of Haemophilus influenzae beta-lactamase: rapid presumptive test for ampicillin resistance.
作者信息
Catlin B W
出版信息
Antimicrob Agents Chemother. 1975 Mar;7(3):265-70. doi: 10.1128/AAC.7.3.265.
Abstract
Strains of Haemophilus influenzae type b sporadically isolated from clinical specimens are ampicillin resistant due to production of a beta-lactamase. This enzyme which inactivates ampicillin and penicillin G is not produced by ampicillin-susceptible strains. Various characteristics of beta-lactamase production and ampicillin resistance of three H. influenzae type b isolates were investigated. A sensitive iodometric test was employed to detect beta-lactamase; positive results were obtained in 5 min with 10(9) bacteria taken from cultures on a nutritionally adequate agar medium. This simple chemical test will enable the hospital laboratory to obtain presumptive evidence of ampicillin resistance on the same day that H. influenzae is isolated.
摘要
从临床标本中偶尔分离出的b型流感嗜血杆菌菌株因产生β-内酰胺酶而对氨苄西林耐药。这种使氨苄西林和青霉素G失活的酶不是对氨苄西林敏感的菌株产生的。对三株b型流感嗜血杆菌分离株的β-内酰胺酶产生及氨苄西林耐药的各种特征进行了研究。采用灵敏的碘量法检测β-内酰胺酶；从营养充足的琼脂培养基上的培养物中取10⁹个细菌，5分钟内即可得到阳性结果。这种简单的化学检测方法可使医院实验室在分离出流感嗜血杆菌的同一天获得氨苄西林耐药的初步证据。
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