β-内酰胺酶阳性和阴性氨苄西林耐药流感嗜血杆菌分离株的简易筛选方法。
Simple screening method for beta-lactamase-positive and -negative ampicillin-resistant Haemophilus influenzae isolates.
作者信息
Parr T R, Pai C H, Bryan L E
出版信息
J Clin Microbiol. 1984 Jul;20(1):131-2. doi: 10.1128/jcm.20.1.131-132.1984.
Abstract
A simple, inexpensive method for screening for beta-lactamase-positive and beta-lactamase-negative ampicillin-resistant Haemophilus influenzae isolates was developed. Disks containing 10 micrograms of cloxacillin yielded no zone of inhibition when placed on chocolate agar plates inoculated with beta-lactamase-positive (16 strains) or ampicillin-resistant (greater than or equal to 1 microgram/ml) beta-lactamase-negative (10 strains) H. influenzae, whereas ampicillin-susceptible (less than or equal to 0.5 microgram/ml; 36 strains) H. influenzae almost always (92%) showed a zone of inhibition.
摘要
开发了一种简单、廉价的方法，用于筛选产β-内酰胺酶和不产β-内酰胺酶的耐氨苄西林流感嗜血杆菌分离株。当将含有10微克氯唑西林的纸片置于接种了产β-内酰胺酶（16株）或耐氨苄西林（大于或等于1微克/毫升）且不产β-内酰胺酶（10株）的流感嗜血杆菌的巧克力琼脂平板上时，未产生抑菌圈，而对氨苄西林敏感（小于或等于0.5微克/毫升；36株）的流感嗜血杆菌几乎总是（92%）显示出抑菌圈。
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