Parr T R, Pai C H, Bryan L E
J Clin Microbiol. 1984 Jul;20(1):131-2. doi: 10.1128/jcm.20.1.131-132.1984.
A simple, inexpensive method for screening for beta-lactamase-positive and beta-lactamase-negative ampicillin-resistant Haemophilus influenzae isolates was developed. Disks containing 10 micrograms of cloxacillin yielded no zone of inhibition when placed on chocolate agar plates inoculated with beta-lactamase-positive (16 strains) or ampicillin-resistant (greater than or equal to 1 microgram/ml) beta-lactamase-negative (10 strains) H. influenzae, whereas ampicillin-susceptible (less than or equal to 0.5 microgram/ml; 36 strains) H. influenzae almost always (92%) showed a zone of inhibition.
开发了一种简单、廉价的方法，用于筛选产β-内酰胺酶和不产β-内酰胺酶的耐氨苄西林流感嗜血杆菌分离株。当将含有10微克氯唑西林的纸片置于接种了产β-内酰胺酶（16株）或耐氨苄西林（大于或等于1微克/毫升）且不产β-内酰胺酶（10株）的流感嗜血杆菌的巧克力琼脂平板上时，未产生抑菌圈，而对氨苄西林敏感（小于或等于0.5微克/毫升；36株）的流感嗜血杆菌几乎总是（92%）显示出抑菌圈。
