Reed R
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Curr Opin Cell Biol. 2000 Jun;12(3):340-5. doi: 10.1016/s0955-0674(00)00097-1.
The pre-mRNA splicing machinery consists of five small nuclear RNAs (U1, U2, U4, U5 and U6) and more than fifty proteins. Over the past year, important advances have been made in understanding how these factors function to achieve fidelity in splicing. Of particular note were the discoveries that the splicing factor U2AF(35) recognizes the AG dinucleotide at the 3' splice site early in spliceosome assembly, that a DEAD-box ATPase, Prp28, triggers specific rearrangements of the spliceosome, and that the splicing factor hSlu7 functions in the fidelity of AG choice during catalytic step II of splicing.
前体信使核糖核酸剪接机制由五种小核核糖核酸（U1、U2、U4、U5和U6）以及五十多种蛋白质组成。在过去的一年里，在理解这些因子如何发挥作用以实现剪接保真度方面取得了重要进展。特别值得注意的是，发现剪接因子U2AF(35)在剪接体组装早期识别3'剪接位点的AG二核苷酸，一种DEAD盒ATP酶Prp28触发剪接体的特定重排，以及剪接因子hSlu7在剪接催化步骤II中AG选择的保真度方面发挥作用。
