卵胞浆内单精子注射最大受精率的评估。
Assessment of maximal fertilization rates with intracytoplasmic sperm injection.
作者信息
Ma S, Ho Yuen B
机构信息
Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, Canada.
出版信息
J Assist Reprod Genet. 2000 Feb;17(2):80-6. doi: 10.1023/a:1009410331774.
PURPOSE
Maximal fertilization rates following ICSI were assessed using two essential steps: immobilization of sperm and aspiration of oocyte cytoplasm.
METHODS
ICSI procedure was performed for couples (N = 42) in whom the male suffered severe infertility or failure of fertilization in previous IVF cycle using different oocyte cytoplasmic aspiration and sperm immobilization methods. Outcome in four patient groups was measured by oocyte damage, fertilization rate, and pregnancy rate.
RESULTS
Maximal fertilization (90%) were achieved from the group which used immobilization of sperm by hard-touching the tail with a pipette and optimal aspiration of oocyte cytoplasm.
CONCLUSIONS
The results suggest that if the immobilization of sperm and aspiration of oocyte cytoplasm are handled right during ICSI, this procedure can be expected to yield a 90% fertilization rate.
目的
采用两个关键步骤评估卵胞浆内单精子注射（ICSI）后的最大受精率，即精子固定和卵母细胞胞质抽吸。
方法
对男性患有严重不育症或在先前体外受精（IVF）周期中受精失败的夫妇（N = 42）进行ICSI操作，使用不同的卵母细胞胞质抽吸和精子固定方法。通过卵母细胞损伤、受精率和妊娠率来衡量四个患者组的结果。
结果
通过用移液管轻触尾部固定精子并进行最佳的卵母细胞胞质抽吸的组实现了最大受精率（90%）。
结论
结果表明，如果在ICSI过程中正确处理精子固定和卵母细胞胞质抽吸，该操作有望产生90%的受精率。
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