Boyd D A, Conly J, Dedier H, Peters G, Robertson L, Slater E, Mulvey M R
Canadian Science Centre for Human and Animal Health, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Winnipeg, Manitoba.
J Clin Microbiol. 2000 Jun;38(6):2392-4. doi: 10.1128/JCM.38.6.2392-2394.2000.
A single vanD-containing Enterococcus faecium strain (N97-330) was isolated in Canada. The vanD-containing region was cloned and sequenced. Although the proteins have more than 96% identity to a previously described vanD region in BM4339, the vanS(D) gene contains a frameshift mutation that leads to a predicted truncated protein. Furthermore, sequence analysis of the ddl gene revealed the presence of an IS982-like element (ISEfm1) which interrupted the D-Ala-D-Ala ligase. This suggested the constitutive expression of the vanD operon, which was confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated that BM4339 was not related to N97-330 (>15 band differences). Both strains contained multiple copies of the IS982-like element.
在加拿大分离出一株含vanD的粪肠球菌菌株（N97 - 330）。对含vanD的区域进行了克隆和测序。尽管这些蛋白质与之前在BM4339中描述的vanD区域有超过96%的同一性，但vanS(D)基因包含一个移码突变，导致预测的截短蛋白。此外，ddl基因的序列分析显示存在一个类似IS982的元件（ISEfm1），它中断了D - 丙氨酸 - D - 丙氨酸连接酶。这表明vanD操纵子的组成型表达，这一点得到了证实。脉冲场凝胶电泳指纹图谱显示BM4339与N97 - 330无关（>15条带差异）。两株菌株都含有多个类似IS982的元件拷贝。
