Cordier G, Samarut C, Brochier J, Revillard J P
Scand J Immunol. 1976;5(3):233-42. doi: 10.1111/j.1365-3083.1976.tb00274.x.
The effector cell(s) in human antibody-dependent cell cytotoxicity (ADCC), with antibody-coated chickens erythrocytes as targets, was studied by comparison of cell suspensions from various lymphoid organs and by means of various cell fractionation methods. Effector cells (K) were found mostly in peripheral blood, spleen, and bone marrow but not in tonsils, lymph nodes, and thymus. Effector cells bear Fc receptors and can form EA rosettes with the antibody-coated target cells. About 1,5% peripheral blood lymphocytes can form 'high-avidity' EA rosettes with targets coated at low antiserum concentration. Most of the effector cells belong to this small subset, as shown by experiments of selective depletion. Removal of most monocytes, T cells, or B cells from, or addition of T-cell-specific antiserum to, the effector cell suspensions did not affect ADCC. Effector cells in this model of ADCC therefore lack the conventional B- or T-cell markers but at least some of them are likely to bear C3 receptors.
以包被抗体的鸡红细胞为靶标，通过比较来自各种淋巴器官的细胞悬液并采用各种细胞分级分离方法，对人抗体依赖细胞介导的细胞毒性作用（ADCC）中的效应细胞进行了研究。效应细胞（K）主要存在于外周血、脾脏和骨髓中，而扁桃体、淋巴结和胸腺中则没有。效应细胞带有Fc受体，并且能与包被抗体的靶细胞形成EA花环。约1.5%的外周血淋巴细胞能与低抗血清浓度包被的靶标形成“高亲和力”EA花环。如选择性去除实验所示，大多数效应细胞属于这一较小的亚群。从效应细胞悬液中去除大多数单核细胞、T细胞或B细胞，或向其中加入T细胞特异性抗血清，均不影响ADCC。因此，在这个ADCC模型中的效应细胞缺乏传统的B细胞或T细胞标志物，但它们中至少有一些可能带有C3受体。
