Brochier J, Samarut C, Bich-Thuy L T, Revillard J P
Immunology. 1978 Nov;35(5):827-35.
Mononuclear cells from eighty-seven human tonsils and fourteen adenoids were examined for T and B cells and their subpopulations. Forty six percent of the tonsil cells were killed in the presence of C by an antiserum specific for T cells and 41.7% were able to form E rosettes. Among them 58% were `active' E rosettes and 24% possessed Fc μ receptors as compared to 35 and 37%, respectively, in peripheral blood. Fifty percent of cells were detected by an antiserum recognizing B cells and monocytes; most of them bore surface immunoglobulins and complement receptors. None of the cells bound chicken erythrocytes coated with rabbit IgG. Only 1 to 3% of monocytes were detected by the peroxidase staining. Significant variations depending on the age of the donors were noticed: T cells increased from 42% in infants to 54.6% in adults whereas the B cells dropped from 56.2 to 38.5%. Similar distributions of cells were found in adenoids and tonsils of donors of the same age. About 2 to 3% of tonsil cells contained cytoplasmic immunoglobulins, more than one third of them being of the IgG class. The proliferative response of adenoid or tonsil cells to Con A, PWM and NWSM was similar to that of blood lymphocytes although their relative numbers of T and B cells were very different, whereas their response to PHA and ALG was slightly lower.
对来自87个人类扁桃体和14个腺样体的单核细胞进行了T细胞、B细胞及其亚群的检测。在补体存在的情况下，一种针对T细胞的抗血清可杀死46%的扁桃体细胞，41.7%的细胞能够形成E花环。其中，58%为“活性”E花环，24%的细胞具有Fcμ受体，而外周血中相应的比例分别为35%和37%。50%的细胞可被一种识别B细胞和单核细胞的抗血清检测到；其中大多数细胞带有表面免疫球蛋白和补体受体。没有细胞能结合用兔IgG包被的鸡红细胞。通过过氧化物酶染色仅检测到1%至3%的单核细胞。观察到细胞比例因供体年龄而异：T细胞从婴儿期的42%增加到成年期的54.6%，而B细胞则从56.2%降至38.5%。在同一年龄供体的腺样体和扁桃体中发现了类似的细胞分布。约2%至3%的扁桃体细胞含有细胞质免疫球蛋白，其中超过三分之一为IgG类。腺样体或扁桃体细胞对刀豆蛋白A、美洲商陆有丝分裂原和新城疫病毒可溶性抗原的增殖反应与血液淋巴细胞相似，尽管它们的T细胞和B细胞相对数量差异很大，而它们对植物血凝素和抗淋巴细胞球蛋白的反应略低。
