Cho Y J, Oh Y H, Abe C, Homma J Y, Usui M, Matuhasi T
Jpn J Exp Med. 1978 Dec;48(6):491-6.
IgE antibody production to exoenzymes and common antigen (OEP) of P. aeruginosa was studied in mice that were injected with the antigens incorporated into water-in-oil-in-water (w/o/w) emulsion or Al(OH)3 gel. OEP and protease toxoid (PT) elicited IgE antibody response but elastase toxoid (ET) did not. Capacity of the OEP to produce IgE antibody was reduced remarkably by it's protease treatment, which suggested that the capacity lies in the protein portion of OEP. The w/o/w emulsion was less effective than Al(OH)3 gel in adjuvanticity to elicit IgE antibody response, but the emulsion enhanced the IgM and/or IgG antibody response to PT and maintained a constant level for a long period. These findings may suggest that IgE antibody response to some components of P. aeruginosa could be induced in man. High serum level of IgE was observed in some cases of cystic fibrosis caused by P. aeruginosa infection, although the IgE antibody activity has not yet been determined.
在注射了掺入水包油包水（w/o/w）乳剂或氢氧化铝凝胶中的抗原的小鼠中，研究了针对铜绿假单胞菌外酶和共同抗原（OEP）的IgE抗体产生情况。OEP和蛋白酶类毒素（PT）引发了IgE抗体反应，但弹性蛋白酶类毒素（ET）未引发。OEP产生IgE抗体的能力通过其蛋白酶处理而显著降低，这表明该能力存在于OEP的蛋白质部分。w/o/w乳剂在引发IgE抗体反应的佐剂作用方面不如氢氧化铝凝胶有效，但该乳剂增强了对PT的IgM和/或IgG抗体反应，并在很长一段时间内维持恒定水平。这些发现可能表明，在人类中可诱导对铜绿假单胞菌某些成分的IgE抗体反应。在一些由铜绿假单胞菌感染引起的囊性纤维化病例中观察到高血清IgE水平，尽管尚未确定IgE抗体活性。
