Hirao Y, Homma J Y
Jpn J Exp Med. 1978 Feb;48(1):41-51.
The effectiveness of immunizing mice with protease toxoid (PT) or elastase toxoid (ET) on the corneal ulcerization due to either protease or elastase were investigated. Consequently, mice immunized with either PT or ET were protected from corneal ulcers experimentally induced by the homologous enzyme, either protease or elastase. Similarly, two kinds of rabbit immune sera, anti-PT and anti-ET, were found to prevent corneal ulcers by the homologous enzyme. Then, the therapeutic effects of vaccination with a single or mixed vaccine consisting of one, two or three components, i.e., PT, ET and/or the common antigen (OEP) of Pseudomonas aeruginosa, were examined on corneal ulcers in mice produced by live cultures of the bacteria. For the same purpose, administration of a single or combined rabbit immune sera against PT, ET and OEP was conducted. As a result, vaccination with the three-component-mixed vaccine or administration with rabbit immune sera combined with anti-PT and anti-ET sera in addition to anti-OEP serum, were found to be the most effective in preventing corneal ulceration as well as in treating corneal ulcers in mice.
研究了用蛋白酶类毒素（PT）或弹性蛋白酶类毒素（ET）免疫小鼠对由蛋白酶或弹性蛋白酶引起的角膜溃疡的效果。结果，用PT或ET免疫的小鼠对由同源酶（蛋白酶或弹性蛋白酶）实验诱导的角膜溃疡具有保护作用。同样，发现两种兔免疫血清，即抗PT和抗ET，可预防同源酶引起的角膜溃疡。然后，研究了用由一种、两种或三种成分组成的单一或混合疫苗，即PT、ET和/或铜绿假单胞菌的共同抗原（OEP），对由该细菌活培养物引起的小鼠角膜溃疡的治疗效果。出于同样的目的，进行了针对PT、ET和OEP的单一或联合兔免疫血清的给药。结果发现，接种三组分混合疫苗或除抗OEP血清外还联合抗PT和抗ET血清的兔免疫血清给药，在预防小鼠角膜溃疡以及治疗角膜溃疡方面最有效。
