Kudchodkar B J, Wilson J, Lacko A, Dory L
Department of Molecular Biology & Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Arterioscler Thromb Vasc Biol. 2000 Jun;20(6):1637-43. doi: 10.1161/01.atv.20.6.1637.
We studied the effect of hyperbaric oxygen (HBO) treatment on the extent of diet-induced accumulation of lipid oxidation products in rabbit plasma and tissues, on plasma paraoxonase activity, and on the extent of progression and regression of atherosclerotic lesions in the rabbit aorta. HBO treatment of cholesterol-fed rabbits dramatically reduces the development of arterial lesions despite having little or no effect on plasma or individual lipoprotein cholesterol concentrations. Compared with no treatment in cholesterol-fed animals, HBO treatment also substantially reduces the accumulation of lipid oxidation products (conjugated dienes, trienes, and thiobarbituric acid-reactive substances) in plasma, in the low density lipoprotein and high density lipoprotein fractions of plasma, in the liver, and in the aortic tissues. In addition, HBO treatment prevents the decrease in plasma paraoxonase activity observed in rabbits fed cholesterol-rich diets. Similarly, in regression studies, HBO treatment has no effect on the rate of plasma (or lipoprotein) cholesterol decline but significantly accelerates aortic lesion regression compared with no treatment. Direct measures of aortic cholesterol content support these morphological observations. On the basis of these results, we conclude that repeated, but relatively short, exposure to HBO induces an antioxidant defense mechanism(s) that is responsible for retarding the development or accelerating the regression of atherosclerotic lesions.
我们研究了高压氧（HBO）治疗对兔血浆和组织中饮食诱导的脂质氧化产物积累程度、血浆对氧磷酶活性以及兔主动脉粥样硬化病变进展和消退程度的影响。对喂食胆固醇的兔子进行HBO治疗，尽管对血浆或单个脂蛋白胆固醇浓度几乎没有影响，但能显著减少动脉病变的发展。与未对喂食胆固醇的动物进行治疗相比，HBO治疗还能大幅减少血浆、血浆低密度脂蛋白和高密度脂蛋白组分、肝脏以及主动脉组织中脂质氧化产物（共轭二烯、三烯和硫代巴比妥酸反应性物质）的积累。此外，HBO治疗可防止喂食富含胆固醇饮食的兔子出现血浆对氧磷酶活性降低的情况。同样，在消退研究中，HBO治疗对血浆（或脂蛋白）胆固醇下降速率没有影响，但与未治疗相比，能显著加速主动脉病变的消退。对主动脉胆固醇含量的直接测量结果支持了这些形态学观察结果。基于这些结果，我们得出结论，反复但相对短暂地暴露于HBO会诱导一种抗氧化防御机制，该机制负责延缓动脉粥样硬化病变的发展或加速其消退。
