Liu D, Coloe S, Baird R, Pedersen J
Melbourne Pathology, 32 Smith Street, Collingwood, Victoria 3066, Australia.
J Med Microbiol. 2000 Jun;49(6):493-497. doi: 10.1099/0022-1317-49-6-493.
Infection of the keratinised tissues (skin, hair and nails) in man and animals by keratinophilic fungi (dermatophytes) results in dermatophytosis (also known as tinea or ringworm). As conventional laboratory procedures for the identification of dermatophytes are either slow or lack specificity, improved diagnostic methods are required. The application of nucleic acid amplification technology has made rapid and precise identification of dermatophytes possible. Recent studies have shown that when one of the four random primers (OPAA11, OPD18, OPAA17 and OPU15) was used in arbitrarily primed PCR (AP-PCR), up to 20 of the 25 dermatophyte species or subspecies under investigation could be distinguished on the basis of characteristic band patterns detected in agarose gel electrophoresis. A combination of two random primers (OPD18 and OPAA17) used in separate reaction tubes identified 23 of the 25 dermatophyte species or subspecies examined. AP-PCR provides a rapid and practical tool for identification of dermatophyte isolates that is independent of morphological and biochemical characteristics and thus enhances laboratory diagnosis of dermatophytosis.
嗜角质真菌（皮肤癣菌）感染人和动物的角质化组织（皮肤、毛发和指甲）会导致皮肤癣菌病（也称为癣或圆癣）。由于用于鉴定皮肤癣菌的传统实验室方法要么耗时较长，要么缺乏特异性，因此需要改进诊断方法。核酸扩增技术的应用使得快速、准确地鉴定皮肤癣菌成为可能。最近的研究表明，当在任意引物PCR（AP-PCR）中使用四种随机引物（OPAA11、OPD18、OPAA17和OPU15）之一时，在所研究的25种皮肤癣菌物种或亚种中，多达20种可以根据琼脂糖凝胶电泳中检测到的特征条带模式进行区分。在单独的反应管中使用两种随机引物（OPD18和OPAA17）的组合，鉴定出了所检测的25种皮肤癣菌物种或亚种中的23种。AP-PCR为鉴定皮肤癣菌分离株提供了一种快速实用的工具，该工具独立于形态学和生化特征，从而增强了皮肤癣菌病的实验室诊断。
