Kopersztych S, Rezkallah M T, Miki S S, Naspitz C K, Mendes N F
Cancer. 1976 Sep;38(3):1149-54. doi: 10.1002/1097-0142(197609)38:3<1149::aid-cncr2820380316>3.0.co;2-x.
Cell-mediated immunity (CMI) was evaluated in 82 patients with non-lymphoid tumors by in vivo and in vitro methods. These included skin test with ubiquitous antigens, 2,4 dinitrochlorobenzene (DNCB) sensitization, determination of T and B peripheral blood lymphocytes, and lymphocyte response to phytohemagglutinin (PHA). The patients were divided into two groups: those with localized and those with disseminated disease (LD and DD). The patients with LD showed no significant differences in CMI when compared with normal controls. The patients with DD showed various defects in CMI when compared with controls and patients with LD. There were significant differences in the response to DNCB, and streptokinase-streptodornase (SK-SD) was the most discriminative of the skin-test antigens. The response to PHA was greatly depressed in patients with DD, whether in the presence of autologous or homologous plasma; in some patients a factor inhibiting to blastogenesis was detected in the serum. In patients with DD, a T-cell lymphopenia was observed. These data showed a correlation between immunocompetence and clinical stage.
采用体内和体外方法对82例非淋巴细胞肿瘤患者的细胞介导免疫（CMI）进行了评估。这些方法包括用常见抗原进行皮肤试验、2,4-二硝基氯苯（DNCB）致敏、测定外周血T和B淋巴细胞以及淋巴细胞对植物血凝素（PHA）的反应。患者被分为两组：局限性疾病组和播散性疾病组（LD和DD）。与正常对照组相比，LD患者的CMI无显著差异。与对照组及LD患者相比，DD患者的CMI存在多种缺陷。对DNCB的反应存在显著差异，链激酶-链道酶（SK-SD）是皮肤试验抗原中最具鉴别力的。DD患者对PHA的反应无论在有自体血浆还是同源血浆存在时均显著降低；在一些患者的血清中检测到一种抑制细胞增殖的因子。在DD患者中，观察到T细胞淋巴细胞减少。这些数据表明免疫能力与临床分期之间存在相关性。
