Lifschytz E, Green M M
Mol Gen Genet. 1979 Mar 20;171(2):153-9. doi: 10.1007/BF00270001.
A model system for the identification of presumptive overproducing mutations from among visible dominant mutations in D. melanogaster is described. An overproducing mutation is expected if a dominant mutation is readily reverted by gene deletion and if gene deletions suppress the expression of the original dominant mutation in flies heterozygous for the deletion. The Beadex (1:59.4) mutations are shown to satisfy these requirements, since a Bx dominant mutations is reverted by induced deletion [Df(Bx)/+) is wild type], and is also suppressed in trans by such a deletion [(Bx/Df)Bx) is wild type]. In addition, all 13 mutations recovered as Bx reversions or suppressors were associated with recessive held up (hdp) mutations allelic inter se, but not allelic to any known hdp gene. One such hdp mutations does not function as an independent dominant suppressor of Bx, is not always associated with Bx deletion, and in the latter situation is readily separable from Bx. We suggest that it functions as a Bx deletion, and may therefore represent the structural gene which is cis-regulated by the overproducing Bx mutations.
本文描述了一种用于从黑腹果蝇的可见显性突变中鉴定推定的过量产生突变的模型系统。如果显性突变容易被基因缺失回复，并且基因缺失抑制了缺失杂合子果蝇中原始显性突变的表达，那么就可以预期存在过量产生突变。结果表明，Beadex（1:59.4）突变满足这些要求，因为Bx显性突变可被诱导缺失回复[Df(Bx)/+)为野生型]，并且在反式中也被这种缺失抑制[(Bx/Df)Bx)为野生型]。此外，作为Bx回复或抑制子回收的所有13个突变都与隐性阻滞（hdp）突变相关，这些突变彼此等位，但与任何已知的hdp基因不等位。其中一个这样的hdp突变不作为Bx的独立显性抑制子起作用，并不总是与Bx缺失相关，并且在后一种情况下很容易与Bx分离。我们认为它作为Bx缺失起作用，因此可能代表由过量产生的Bx突变顺式调控的结构基因。
