Kohashi O, Pearson C M, Shimono T, Kotani S
Int Arch Allergy Appl Immunol. 1976;51(4):451-61. doi: 10.1159/000231619.
Cell walls of Mycobacterium smegmatis were able to produce much more severe arthritis in rats than the delipidated cells, whereas cell envelope and cell membrane fractions were unable to produce the disease. The lysozyme-solubilized product was able to produce mild disease with only 30% of incidence with an optimum dose, whereas the higher and the lower doses did not produce the disease. The rats immunized with cell envelope, cell membrane fraction and nonarthritogenic doses of lysozyme-solubilized product were protected against the subsequent homologous and heterologous challenge of delipidated cells. It was discussed that this preventative effect can be the result of antigenic competition between the arthritogenic and nonarthritogenic components of M. smegmatis. On the other hand, all the fractions separated here were able to serve as an immunoadjuvant in terms of inducing delayed hypersensitivity to ovalbumin in guinea pigs.
耻垢分枝杆菌的细胞壁在大鼠中比脱脂细胞能引发严重得多的关节炎，而细胞包膜和细胞膜组分则无法引发该病。溶菌酶溶解产物在最佳剂量时能引发轻度疾病，发病率仅为30%，而较高和较低剂量均未引发疾病。用细胞包膜、细胞膜组分和非致关节炎剂量的溶菌酶溶解产物免疫的大鼠，可抵御随后脱脂细胞的同源和异源攻击。据讨论，这种预防作用可能是耻垢分枝杆菌致关节炎和非致关节炎成分之间抗原竞争的结果。另一方面，此处分离的所有组分在诱导豚鼠对卵清蛋白的迟发型超敏反应方面都能作为免疫佐剂。
