Nistri A
Naunyn Schmiedebergs Arch Pharmacol. 1976 Oct;295(1):89-94. doi: 10.1007/BF00509778.
The in vitro frog spinal cord has been used to study acetylcholine (ACh) release and spinal root potentials. The preparation bathed in an eserine-containing medium spontaneously released ACh into the bathing fluid. This release was enhanced by atropine in a dose-related and long-lasting manner and transiently by oxotremorine. The release rate of ACh was increased by low frequency ventral root stimulation; this increase was found to be proportionally the same after application of atropine. Oxotremorine did not modify the elctrically-evoked ACh release but blocked or reduced the effect of atropine. It is concluded that the stimulatory action of atropine on ACh output cannot be entirely explained by an interaction of atropine with presynaptic cholinergic receptors and that other indirect mechanisms (via interneurones) may play a role in this effect.
体外青蛙脊髓已被用于研究乙酰胆碱（ACh）的释放和脊髓根电位。浸泡在含有毒扁豆碱的培养基中的标本会自发地将ACh释放到浴液中。阿托品以剂量相关且持久的方式增强了这种释放，而氧化震颤素则短暂增强了这种释放。低频腹根刺激可提高ACh的释放速率；发现应用阿托品后，这种增加成比例相同。氧化震颤素并未改变电诱发的ACh释放，但阻断或降低了阿托品的作用。得出的结论是，阿托品对ACh输出的刺激作用不能完全用阿托品与突触前胆碱能受体的相互作用来解释，其他间接机制（通过中间神经元）可能在这种作用中发挥作用。
