Amarowicz R, Naczk M, Shahidi F
Department of Human Nutrition, St. Francis Xavier University, Antigonish, P.O. Box 5000, NS B2G 2W5, Canada.
J Agric Food Chem. 2000 Jul;48(7):2755-9. doi: 10.1021/jf9911601.
Cyclone canola hulls were extracted with 70% (v/v) acetone. The dried crude extract was dissolved in ethanol and fractionated on a Sephadex LH-20 column using 95% (v/v) ethanol as the mobile phase. Five major fractions were isolated according to the UV absorption. All fractions exhibited marked antioxidant activity in a beta-carotene-linoleate model system. Fractions I and II showed the best preventive effect against the bleaching of beta-carotene. The scavenging effect of fractions I, III, and V, at 1 mg, on alpha, alpha-diphenyl-beta-picrylhydrazyl (DPPH) radical was 67.4%, 80.7%, and 63.3%, respectively. Fractions II and IV showed weak DPPH scavenging effects. The reducing power of phenolics present in fractions IV and V was greater than that of fractions I-III, and the observed data correlated well (r(2) = 0.937; P = 0.007) with the total content of phenolics present in each fraction.
用70%（v/v）丙酮提取油菜籽旋风分离的外壳。将干燥的粗提取物溶解在乙醇中，并以95%（v/v）乙醇作为流动相在Sephadex LH - 20柱上进行分馏。根据紫外吸收分离出五个主要馏分。在β-胡萝卜素-亚油酸酯模型系统中，所有馏分均表现出显著抗氧化活性。馏分I和II对β-胡萝卜素的漂白具有最佳预防效果。馏分I、III和V在1mg时对α，α-二苯基-β-苦基肼（DPPH）自由基的清除率分别为67.4%、80.7%和63.3%。馏分II和IV表现出较弱的DPPH清除效果。馏分IV和V中酚类物质的还原能力大于馏分I - III，观察数据与各馏分中酚类物质的总含量相关性良好（r(2) = 0.937；P = 0.007）。
