McLaren C, Cheng H, Spicer D L, Tompkins W A
Infect Immun. 1976 Oct;14(4):1014-21. doi: 10.1128/iai.14.4.1014-1021.1976.
Using a semimicromethod with washed whole blood, in vitro lymphocyte responses of rabbits to intradermal infection with vaccinia virus was studied. Peritoneal exudate macrophages were infected with vaccinia in vitro to determine the time of appearance of activated macrophages. After primary infection, an increase in spontaneous incorporation of thymidine by blood cultures was found as early as 2 days postinfection. This effect was at a maximum at 7 to 10 days, with counts up to 100-fold higher than before infection. Incubation of these cultures with concanavalin A showed a marked decrease in stimulation index as compared with normals. Although only a transient stimulation with vaccinia was found during the acute infection, stimulation indexes of 2 to 3 were obtained during convalescence. Macrophages from rabbits early after infection supported vaccinia replication, whereas those at day 6 or later resisted infection. Macrophage resistance persisted for 2 to 3 weeks. The response of lymphocytes from rabbits reinfected with vaccinia after 15 weeks differed, with a small increase in spontaneous thymidine uptake, a smaller depression in concanavalin A stimulation, and a greater specific response to vaccinia. Macrophage activation occurred earlier and persisted for a longer time after secondary infection.
采用一种用洗涤全血的半微量法，研究了兔对痘苗病毒皮内感染的体外淋巴细胞反应。体外将痘苗感染腹腔渗出巨噬细胞以确定活化巨噬细胞出现的时间。初次感染后，早在感染后2天就发现血培养中胸苷的自发掺入增加。这种效应在7至10天达到最大值，计数比感染前高100倍。与正常情况相比，这些培养物与伴刀豆球蛋白A一起孵育显示刺激指数明显降低。虽然在急性感染期间仅发现痘苗的短暂刺激，但在恢复期获得了2至3的刺激指数。感染后早期兔的巨噬细胞支持痘苗复制，而在第6天或更晚的巨噬细胞则抵抗感染。巨噬细胞抗性持续2至3周。15周后再次感染痘苗的兔淋巴细胞反应不同，胸苷自发摄取略有增加，伴刀豆球蛋白A刺激的抑制作用较小，对痘苗的特异性反应更大。二次感染后巨噬细胞活化出现得更早且持续时间更长。
