Sweeney C R, Russell G E
Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348-1692, USA.
J Am Vet Med Assoc. 2000 Jul 1;217(1):54-7. doi: 10.2460/javma.2000.217.54.
To examine total protein concentration and cell counts of sequentially collected samples of CSF to determine whether blood contamination decreases in subsequent samples and whether formulas used to correct nucleated cell count and total protein concentration are accurate.
Case series.
22 horses.
For each horse, 3 or 4 sequential 2-ml samples of CSF were collected from the subarachnoid space in the lumbosacral region into separate syringes, and blood was obtained from the jugular vein. Total protein concentration, nucleated cell count, and RBC counts were determined in all samples.
Among 3 sequential samples, total protein concentration and RBC count were significantly lower in samples 2 and 3, compared with sample 1. Nucleated cell count was significantly lower in sample 3, compared with sample 1. Among 4 sequential samples, total protein concentration and RBC count were significantly lower in samples 2, 3, and 4, compared with sample 1. Nucleated cell count was significantly lower in samples 3 and 4, compared with sample 1. For 3 correction formulas, significant differences in corrected values for nucleated cell count and total protein concentration were detected between sample 1 and sample 3 or 4.
Because iatrogenic blood contamination decreases in sequential CSF samples, a minimum of 3 samples should be collected before submitting the final sample for analysis. Formulas to correct nucleated cell count and total protein concentration are inaccurate and should not be used to correct for blood contamination in CSF samples.
检测脑脊液连续采集样本的总蛋白浓度和细胞计数，以确定后续样本中的血液污染是否减少，以及用于校正有核细胞计数和总蛋白浓度的公式是否准确。
病例系列研究。
22匹马。
对每匹马，从腰荐部蛛网膜下腔连续采集3或4个2毫升脑脊液样本，分别注入不同注射器，并从颈静脉采血。测定所有样本的总蛋白浓度、有核细胞计数和红细胞计数。
在连续3个样本中，与样本1相比，样本2和样本3的总蛋白浓度和红细胞计数显著降低。与样本1相比，样本3的有核细胞计数显著降低。在连续4个样本中，与样本1相比，样本2、样本3和样本4的总蛋白浓度和红细胞计数显著降低。与样本1相比，样本3和样本4的有核细胞计数显著降低。对于3种校正公式，样本1与样本3或样本4之间，有核细胞计数和总蛋白浓度的校正值存在显著差异。
由于连续脑脊液样本中的医源性血液污染减少，在提交最终样本进行分析之前应至少采集3个样本。校正有核细胞计数和总蛋白浓度的公式不准确，不应使用于校正脑脊液样本中的血液污染。
