Department of Animal Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Internal Veterinary Medicine, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
BMC Vet Res. 2021 Sep 9;17(1):302. doi: 10.1186/s12917-021-03007-4.
Diseases of the central nervous system are a well-recognized cause of morbidity and mortality in equine. Collection and analysis of cerebrospinal fluid (CSF) give information about the type and stage of degenerative and inflammatory diseases in central nervous system (CNS). The present research aimed to assess the clinical complications of CSF collections and to establish range values of cytological and biochemical parameters of CSF in adult healthy donkeys (Equus asinus). The CSF samples were collected from fifty healthy donkeys at the lumbosacral (LS) and atlanto-occipital (AO) sites.
Hypothermia, tachycardia, ataxia and recumbency may develop post-puncture. Erythrocytes were noticed in 35 of 50 CSF samples. Total nucleated cell counts ranged from 0 to 6 cells/μL, and lymphocytes predominated the cells (61%). The concentration of glucose (1.2 to 5.3 mmol/L) was lower than that of serum (P < 0.05). The CSF sodium concentration (123 to 160 mmol/L) was approximately like that of serum, but potassium (1.5-3 mmol/L) was lower than that of serum (P < 0.01). Urea concentrations (1.1-2.9 mmol/L) were markedly lower than serum (P < 0.001). Concentrations of CSF total proteins, and albumin ranged from 0.1 to 0.6 g/dL, and from 0.002 to 0.013 g/dL, respectively. The albumin quotient ranged from 0.06 to 0.56.
Transient hypothermia, tachycardia, ataxia and recumbency may develop as clinical complications of CSF puncture procedures. The collection site has no impact on the constituents in CSF. Furthermore, this study presented the range values for normal cytological and biochemical constituents of CSF in donkeys (Equus asinus) that can provide a basis in comparison when evaluating CSF from donkeys with neurologic diseases.
中枢神经系统疾病是马属动物发病率和死亡率的一个公认原因。收集和分析脑脊液（CSF）可提供有关中枢神经系统（CNS）退行性和炎症性疾病类型和阶段的信息。本研究旨在评估 CSF 采集的临床并发症，并确定成年健康驴（Equus asinus）CSF 的细胞和生化参数的范围值。从 50 头健康驴的腰荐（LS）和寰枕（AO）部位采集 CSF 样本。
穿刺后可能会出现体温过低、心动过速、共济失调和卧地。在 50 个 CSF 样本中，有 35 个样本中发现了红细胞。总核细胞计数范围为 0 至 6 个/μL，淋巴细胞为主（61%）。葡萄糖浓度（1.2 至 5.3mmol/L）低于血清（P<0.05）。CSF 钠浓度（123 至 160mmol/L）与血清相似，但钾（1.5-3mmol/L）低于血清（P<0.01）。尿素浓度（1.1-2.9mmol/L）明显低于血清（P<0.001）。CSF 总蛋白和白蛋白浓度分别为 0.1 至 0.6g/dL 和 0.002 至 0.013g/dL，白蛋白商数范围为 0.06 至 0.56。
CSF 穿刺术可能会出现短暂的体温过低、心动过速、共济失调和卧地等临床并发症。采集部位对 CSF 中的成分没有影响。此外，本研究提供了驴（Equus asinus）正常 CSF 细胞学和生化成分的范围值，可为评估神经疾病驴的 CSF 提供依据。
