Roger M, Faucher M C, Forest P, St-Antoine P, Coutlée F
Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Département de Microbiologie Médicale et Infectiologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada.
J Clin Microbiol. 1999 Oct;37(10):3348-9. doi: 10.1128/JCM.37.10.3348-3349.1999.
We investigated the use of PCR as an alternative to culture of fecal samples for detection of vanA-containing Enterococcus faecium during a recent hospital outbreak. Rectal swabs collected consecutively from 223 patients were analyzed by culture with and without enrichment broth and by vanA-specific PCR of enrichment broth samples. Fifty-five specimens were positive for vanA-containing E. faecium by at least one method. The sensitivities of the vanA-specific PCR assay and agar culture with and without enrichment broth were 94.5, 98, and 89%, respectively. All three methods were 100% specific. Final results were obtained much more rapidly by PCR (within 24 to 30 h of specimen submission) than by the culture methods (4 to 5 days). Thus, PCR is an accurate and rapid alternative to culture for detection of vancomycin-resistant enterococci during hospital outbreaks.
在最近一次医院感染暴发期间，我们研究了使用聚合酶链反应（PCR）作为粪便样本培养的替代方法，以检测含vanA基因的屎肠球菌。对连续收集的223例患者的直肠拭子进行分析，采用有无增菌肉汤培养法以及对增菌肉汤样本进行vanA特异性PCR检测。至少一种方法检测出55份标本含vanA基因的屎肠球菌呈阳性。vanA特异性PCR检测法以及有无增菌肉汤的琼脂培养法的敏感度分别为94.5%、98%和89%。三种方法的特异性均为100%。与培养法（4至5天）相比，PCR能更快得出最终结果（标本送检后24至30小时内）。因此，在医院感染暴发期间，PCR是检测耐万古霉素肠球菌的一种准确且快速的培养替代方法。
