Hurley D L, Fauci A S
J Infect Dis. 1975 May;131(5):516-27. doi: 10.1093/infdis/131.5.516.
A model of experimental disseminated candidiasis was developed in the guinea pig; Candida albicans was injected intraperitoneally or intravenously. The kidney was the most severely affected organ, with maximal colony counts of 26,208 plus or minus 9,152 colony-forming units/g of tissue five days after a sublethal inocluation (one-sixth of the 50% lethal dose) of viable organisms. The heart was the next most severely affected organ, and other organs were affected little. Histologic studies confirmed the data from colony counts and showed a progression of inflammation similar to that seen in human disease. A leukocytosis predominantly of mononuclear cells, and to a lesser degree of granulocytes, occurred and was maximal four days after inoculation. This model will provide the framework for a series of future studies of the various factors important in natural host defense against infection with Candida, as well as the effects of various immunosuppressive agents, particularly corticosteroids, on the development and expression of immunity and protection against infection.
在豚鼠身上建立了实验性播散性念珠菌病模型；白色念珠菌通过腹腔内或静脉内注射。肾脏是受影响最严重的器官，在亚致死剂量（50%致死剂量的六分之一）的活生物体接种五天后，每克组织的最大菌落数为26,208±9,152个菌落形成单位。心脏是其次受影响最严重的器官，其他器官受影响较小。组织学研究证实了菌落计数的数据，并显示出与人类疾病中所见相似的炎症进展。出现了以单核细胞为主、粒细胞程度较轻的白细胞增多，接种后四天达到最大值。该模型将为一系列未来研究提供框架，这些研究涉及天然宿主防御念珠菌感染中重要的各种因素，以及各种免疫抑制剂，特别是皮质类固醇，对免疫和抗感染的发育、表达的影响。
