携带对氨基糖苷类抗生素耐药性的R因子的遗传特性。

Gentic properties of an R factor carrying resistance to aminolgycoside antibiotics.

作者信息

Hasuda K, Krcméry V, Iyobe S, Mitsuhashi S

出版信息

J Bacteriol. 1975 Jul;123(1):329-35. doi: 10.1128/jb.123.1.329-335.1975.

DOI:10.1128/jb.123.1.329-335.1975

PMID:1095559

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235723/

Abstract

R factor Rms 151 is an fi+ R factor and belongs to a incompatibility group FII. It carries the genes governing resistance to various aminoglycoside antibiotics, i.e., kanamycin (KM), lividomycin (LV), gentamicin C complex (GM), and 3',4'-dideoxykanamycin B (DKB), in addition to those governing to tetracycline (TC), chloramphenicol (CM), sulfanilamide (SA), and ampicillin (APC). Electron microscopy observation disclosed that the Rms151 deoxyribonucleic acid was a circular form with length of 31.2 mum. A probable circular genetic map of Rms151 was proposed by genetic and biochemical studies, the genes being in the order of -tet-tra-amp-aad-sul-aph-cml-, in which aad and aph confer resistance to KM.GM.DKB by adenylytransferase or resistance to KM.LV by phosphotransferase, respectively.

摘要

R因子Rms 151是一种fi+ R因子，属于不相容群FII。它携带控制对多种氨基糖苷类抗生素耐药性的基因，即卡那霉素（KM）、青紫霉素（LV）、庆大霉素C复合物（GM）和3',4'-双脱氧卡那霉素B（DKB），此外还携带控制对四环素（TC）、氯霉素（CM）、磺胺（SA）和氨苄青霉素（APC）耐药性的基因。电子显微镜观察表明，Rms151脱氧核糖核酸呈环状，长度为31.2微米。通过遗传和生化研究提出了Rms151可能的环状遗传图谱，基因顺序为-tet-tra-amp-aad-sul-aph-cml-，其中aad和aph分别通过腺苷酸转移酶赋予对KM、GM、DKB的耐药性或通过磷酸转移酶赋予对KM、LV的耐药性。