Kawabe H, Inoue M, Mitsuhashi S
Antimicrob Agents Chemother. 1974 Jun;5(6):553-7. doi: 10.1128/AAC.5.6.553.
Three types of Staphylococcus aureus strains were isolated with respect to streptomycin (SM) and spectinomycin (SPC) resistance, namely, SM(r)SPC(r), SM(r)SPC(s), and SM(s)SPC(r) (r, resistant; s, sensitive). Curing experiments and transduction analysis of strain MS7990 (SM(r).SPC(r).EM(r)) (EM, erythromycin) disclosed that the loci governing SM and SPC resistance are different and exist on different nontransferable plasmids (r factor), one plasmid carrying the genes governing SM resistance and another possessing the genes governing resistance to both SPC and EM. Strain MS7990 (SM(r).SPC(r)) inactivated both drugs by adenylylation. Similarly, the SM(r)SPC(s) and SM(s)SPC(r) strains inactivated SM and SPC, respectively, by adenylylation, although the adenylylated positions of both drugs have not been established as yet. The adenylylated SM in staphylococci was shown to be different from 3''-adenylyl-SM, indicating the possibility of the existence of a different enzyme from SM3''-adenylyl transferase demonstrated in Escherichia coli strains.
根据对链霉素（SM）和壮观霉素（SPC）的耐药性，分离出三种金黄色葡萄球菌菌株，即SM(r)SPC(r)、SM(r)SPC(s)和SM(s)SPC(r)（r表示耐药，s表示敏感）。对菌株MS7990（SM(r).SPC(r).EM(r)）（EM为红霉素）进行的消除实验和转导分析表明，控制SM和SPC耐药性的基因座不同，且存在于不同的非转移性质粒（r因子）上，一个质粒携带控制SM耐药性的基因，另一个质粒则拥有控制对SPC和EM耐药性的基因。菌株MS7990（SM(r).SPC(r)）通过腺苷酸化使两种药物失活。同样，SM(r)SPC(s)和SM(s)SPC(r)菌株分别通过腺苷酸化使SM和SPC失活，尽管两种药物的腺苷酸化位置尚未确定。葡萄球菌中腺苷酸化的SM与3''-腺苷酰-SM不同，这表明可能存在一种与在大肠杆菌菌株中发现的SM3''-腺苷酰转移酶不同的酶。
