McGaw L J, Jäger A K, van Staden J
Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
J Ethnopharmacol. 2000 Sep;72(1-2):247-63. doi: 10.1016/s0378-8741(00)00269-5.
Hexane, ethanol and water extracts of plants used by South African traditional healers for treating stomach ailments were screened for antibacterial, anthelmintic and anti-amoebic activities. To evaluate antibacterial activity, the disc-diffusion assay was used against several Gram-positive and Gram-negative species. Minimal inhibitory concentration values were determined with a microdilution assay. Ethanolic extracts showed the greatest activity, and Gram-positive bacteria were the most susceptible microorganisms. The free-living nematode Caenorhabditis elegans was used in two different assays to evaluate anthelmintic activity. A microdilution technique was employed to investigate anti-amoebic activity against the enteropathogenic Entamoeba histolytica. These assays were suitable for the screening of a large number of extracts at one time. Several plants exhibited significant activity against these test organisms.
对南非传统治疗师用于治疗胃部疾病的植物的己烷、乙醇和水提取物进行了抗菌、驱虫和抗阿米巴活性筛选。为评估抗菌活性，采用纸片扩散法对几种革兰氏阳性菌和革兰氏阴性菌进行检测。用微量稀释法测定最低抑菌浓度值。乙醇提取物显示出最大活性，革兰氏阳性菌是最敏感的微生物。采用两种不同的试验方法，利用自由生活线虫秀丽隐杆线虫评估驱虫活性。采用微量稀释技术研究对肠道致病性溶组织内阿米巴的抗阿米巴活性。这些试验方法适合一次性筛选大量提取物。几种植物对这些受试生物表现出显著活性。
