Sims J E, Williams D E, Morrissey P J, Garka K, Foxworthe D, Price V, Friend S L, Farr A, Bedell M A, Jenkins N A, Copeland N G, Grabstein K, Paxton R J
Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101, USA.
J Exp Med. 2000 Sep 4;192(5):671-80. doi: 10.1084/jem.192.5.671.
Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220(+)BP-1(+) pre-B cells.
利用一种由小鼠B细胞系增殖组成的生物测定法，从胸腺基质细胞系中分离出一个基因的cDNA，其产物支持该细胞系的生长。这种因子被称为胸腺基质淋巴细胞生成素（TSLP），是一种由140个氨基酸组成的蛋白质。编码TSLP的基因被定位到小鼠18号染色体上。纯化的重组TSLP在体外支持前B细胞集落的生长，但没有髓系造血活性。TSLP对胎儿胸腺细胞具有协同刺激活性，但在叶浸没培养中不如白细胞介素7有效。将TSLP注射到新生小鼠体内可诱导B220(+)BP-1(+)前B细胞的扩增。
