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1
Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.酶联免疫吸附测定（ELISA）在梅毒血清学诊断中的应用。
Br J Vener Dis. 1975 Aug;51(4):227-31. doi: 10.1136/sti.51.4.227.
2
Enzyme-linked immunosorbent assay in the serodiagnosis of syphilis.酶联免疫吸附测定在梅毒血清学诊断中的应用
Acta Microbiol Acad Sci Hung. 1982;29(4):221-6.
3
Serodiagnosis of syphilis by an enzyme-linked immunosorbent assay for IgG antibodies against the Reiter treponeme flagellum.通过酶联免疫吸附测定法检测针对赖特疏螺旋体鞭毛的IgG抗体进行梅毒血清学诊断。
Scand J Immunol. 1982 Apr;15(4):341-8. doi: 10.1111/j.1365-3083.1982.tb00657.x.
4
Enzyme linked immunosorbent assays with Treponema pallidum or axial filament of T phagedenis biotype Reiter as antigen: evaluation as screening tests for syphilis.以梅毒螺旋体或杜克雷噬菌体型赖特氏轴向丝为抗原的酶联免疫吸附试验：作为梅毒筛查试验的评估
Genitourin Med. 1986 Dec;62(6):367-72. doi: 10.1136/sti.62.6.367.
5
Development and evaluation of a monoclonal antibody inhibition enzyme linked immunosorbent assay to diagnose syphilis.一种用于诊断梅毒的单克隆抗体抑制酶联免疫吸附测定法的开发与评估
Genitourin Med. 1989 Oct;65(5):308-15. doi: 10.1136/sti.65.5.308.
6
[Serologic diagnosis of syphilis; method and interpretation].[梅毒的血清学诊断；方法与解读]
Ned Tijdschr Geneeskd. 1977 Jun 18;121(25):1025-34.
7
[Practical significance of syphilis serodiagnosis].[梅毒血清学诊断的实际意义]
Fortschr Med. 1978 Nov 9;96(42):2142-6.
8
[Application of an immunoenzyme test (ELISA) with a purified treponemal antigen (Reiter) in the serodiagnosis of syphilis].[应用纯化梅毒螺旋体抗原（赖特氏）的免疫酶试验（酶联免疫吸附测定）进行梅毒血清学诊断]
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1985 Oct-Dec;30(4):363-8.
9
Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis.用于检测人类梅毒特异性IgM抗体的微酶联免疫吸附测定法。
Br J Vener Dis. 1983 Jun;59(3):151-6. doi: 10.1136/sti.59.3.151.
10
[Passive hemagglutination test in the serodiagnosis of syphilis].[梅毒血清诊断中的被动血凝试验]
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1979 Apr-Jun;24(2):109-13.

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1
tANCHOR cell-based ELISA approach as a surrogate for antigen-coated plates to monitor specific IgG directed to the SARS-CoV-2 receptor-binding domain.基于锚定细胞的酶联免疫吸附测定法作为抗原包被板的替代方法，用于监测针对严重急性呼吸综合征冠状病毒2受体结合域的特异性免疫球蛋白G。
Biol Methods Protoc. 2024 Jan 19;9(1):bpae001. doi: 10.1093/biomethods/bpae001. eCollection 2024.
2
CDC Laboratory Recommendations for Syphilis Testing, United States, 2024.美国疾病预防控制中心 2024 年梅毒检测实验室推荐意见
MMWR Recomm Rep. 2024 Feb 8;73(1):1-32. doi: 10.15585/mmwr.rr7301a1.
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The endemic treponematoses.地方性梅毒螺旋体病。
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It is time to use treponema-specific antibody screening tests for diagnosis of syphilis.现在是时候使用梅毒螺旋体特异性抗体筛查试验来诊断梅毒了。
J Clin Microbiol. 2012 Jan;50(1):2-6. doi: 10.1128/JCM.06347-11. Epub 2011 Nov 16.
5
Comparative evaluation of nine different enzyme-linked immunosorbent assays for determination of antibodies against Treponema pallidum in patients with primary syphilis.九种不同酶联免疫吸附测定法用于检测一期梅毒患者抗梅毒螺旋体抗体的比较评估。
J Clin Microbiol. 2000 Mar;38(3):1279-82. doi: 10.1128/JCM.38.3.1279-1282.2000.
6
Evaluation of a Treponema pallidum enzyme immunoassay as a screening test for syphilis.梅毒螺旋体酶免疫测定作为梅毒筛查试验的评估
Clin Diagn Lab Immunol. 1994 Jul;1(4):477-81. doi: 10.1128/cdli.1.4.477-481.1994.
7
Laboratory diagnosis and interpretation of tests for syphilis.梅毒检测的实验室诊断与解读
Clin Microbiol Rev. 1995 Jan;8(1):1-21. doi: 10.1128/CMR.8.1.1.
8
Evaluation of immunoglobulin G enzyme immunoassay for serodiagnosis of yaws.用于雅司病血清学诊断的免疫球蛋白G酶免疫测定法的评估
J Clin Microbiol. 1995 Jul;33(7):1875-8. doi: 10.1128/jcm.33.7.1875-1878.1995.
9
Comparison of CAPTIA syphilis G enzyme immunoassay with rapid plasma reagin test for detection of syphilis.CAPTIA梅毒G酶免疫测定法与快速血浆反应素试验在梅毒检测中的比较。
J Clin Microbiol. 1995 Jul;33(7):1829-31. doi: 10.1128/jcm.33.7.1829-1831.1995.
10
Evaluation of the microenzyme-linked immunosorbent assay with Treponema pallidum antigen.用梅毒螺旋体抗原评估微酶联免疫吸附测定法。
J Clin Microbiol. 1982 Apr;15(4):630-4. doi: 10.1128/jcm.15.4.630-634.1982.

本文引用的文献

1
Experiences with the treponemal immobilization test in the Netherlands.
Antonie Van Leeuwenhoek. 1955;21(3):331-6. doi: 10.1007/BF02543829.
2
Isolation and purification of the protein antigen of the Reiter treponeme; a study of its serologic reactions.赖特密螺旋体蛋白抗原的分离与纯化；其血清学反应的研究。
Am J Syph Gonorrhea Vener Dis. 1953 Mar;37(2):137-50.
3
Immunoassay using antigen-enzyme conjugates.使用抗原-酶偶联物的免疫测定法。
FEBS Lett. 1971 Jun 24;15(3):232-236. doi: 10.1016/0014-5793(71)80319-8.
4
Performance and use of the Reiter protein complement-fixation (RPCF) test.赖特蛋白补体结合（RPCF）试验的性能与应用。
Br J Vener Dis. 1966 Mar;42(1):42-3. doi: 10.1136/sti.42.1.42.
5
The antigenic structure of Treponema pallidum, Nichols strain. I. The demonstration, nature and location of specific and shared antigens.梅毒螺旋体Nichols株的抗原结构。I. 特异性抗原和共同抗原的证实、性质及定位
J Immunol. 1966 Mar;96(3):450-6.
6
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.

酶联免疫吸附测定（ELISA）在梅毒血清学诊断中的应用。

Application of the enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of syphilis.

作者信息

Veldkamp J, Visser A M

出版信息

Br J Vener Dis. 1975 Aug;51(4):227-31. doi: 10.1136/sti.51.4.227.

DOI:10.1136/sti.51.4.227
PMID:1098730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1046555/
Abstract

The enzyme-linked immunosorbent assay (ELISA) technique, using an ultrasonicate of Treponema pallidum as antigen, has been evaluated as a serological test for syphilis. It is concluded that the test is simple, reliable, and relatively quick and that its sensitivity in all stages of syphilis is equal to the FTAABS test. Because its specificity is probably also high, ELISA might be used in future as a first-line screening test for the serodiagnosis of syphilis.

摘要

以梅毒螺旋体超声提取物为抗原的酶联免疫吸附测定（ELISA）技术已被评估为梅毒的血清学检测方法。得出的结论是，该检测方法简单、可靠且相对快速，并且其在梅毒各阶段的敏感性与荧光密螺旋体抗体吸收试验（FTA-ABS试验）相当。由于其特异性可能也很高，ELISA未来可能用作梅毒血清学诊断的一线筛查试验。