基于核酸序列扩增法与分支DNA检测法在定量血浆中HIV RNA载量方面的比较。

Comparison between a nucleic acid sequence-based amplification and branched DNA test for quantifying HIV RNA load in blood plasma.

作者信息

Berndt C, Müller U, Bergmann F, Schmitt U, Kaiser R, Müller C

机构信息

Institute of Laboratory Medicine and Pathobiochemistry, University Hospital Charité, Campus Virchow-Klinikum, Augustenburger Platz 1, D-13353, Berlin, Germany.

出版信息

J Virol Methods. 2000 Sep;89(1-2):177-81. doi: 10.1016/s0166-0934(00)00225-1.

DOI:10.1016/s0166-0934(00)00225-1

PMID:10996651

Abstract

HIV RNA was quantified in blood plasma from 209 patients and in control specimen comparing the NucliSens HIV-1 QT test (Organon Teknika), which is based on the nucleic acid sequence amplification procedure, and the Quantiplex 3.0 test (Bayer), which uses hybridization signal enhancement by branched DNA (bDNA) probes. A highly significant correlation (P=0.01) was found between the two methods with 88% of the samples showing similar results. In cases of discrepant findings, higher virus load was observed with either test (14xNASBA>bDNA; 12xbNDA>NASBA). Differences could neither be related to clinical features nor to divergent virus subtypes. Standard preparations containing 35000 and 222000 copies were quantified with intra-assay coefficients of variation of <20% using both methods. A preparation of 192 copies was measured with lower precision by both tests, yet was detected more reliably by the bDNA method.

摘要

对209例患者的血浆以及对照样本中的HIV RNA进行了定量分析，比较了基于核酸序列扩增程序的NucliSens HIV-1 QT检测法（奥加农泰尼克公司）和使用分支DNA（bDNA）探针进行杂交信号增强的Quantiplex 3.0检测法（拜耳公司）。两种方法之间发现高度显著相关性（P = 0.01），88%的样本结果相似。在结果不一致的情况下，两种检测方法均观察到较高的病毒载量（14次核酸序列扩增法>分支DNA法；12次分支DNA法>核酸序列扩增法）。差异既与临床特征无关，也与不同的病毒亚型无关。使用两种方法对含有35000和222000拷贝的标准制剂进行定量分析，批内变异系数均<20%。两种检测方法对192拷贝的制剂测量精度较低，但分支DNA法检测更可靠。