Duan S B, Liu F Y, Luo J A, Wu H W, Liu R H, Peng Y M, Yang X L
Department of Medicine, the Second Affiliated Hospital, Hunan Medical University, Changsha, P. R. of China.
Acta Radiol. 2000 Sep;41(5):503-7. doi: 10.1080/028418500127345794.
To evaluate the nephrotoxicity of high- and low-osmolar contrast media (HOCM, LOCM) on kidneys in Sprague-Dawley rats. The protective role of amlodipine was studied.
Forty rats of both sexes were randomly divided into 5 groups (n=8/group) and glycerine for inducing renal failure was given to all rats except controls.
In diatrizoate-injected rats, blood urea nitrogen (BUN) and serum creatinine (SCr) were increased; levels of phospholipase A2 (PLA2), lipid peroxide (LPO) and calcium were also increased in renal tissues. There was no significant difference between LOCM (iohexol) animals and glycerol controls either in the renal levels of PLA2, LPO and calcium or in the levels of BUN and SCr. The histologic changes were milder in the LOCM animals than in the HOCM animals. In the group pretreated with amlodipine, no increase in the levels of BUN or SCr was discovered and the renal content of PLA2, LPO and calcium were significantly lower than in the HOCM group; the renal injuries induced by diatrizoate were alleviated.
The HOCM, diatrizoate, was more toxic to rat kidneys than the LOCM iohexol; PLA2, LPO and calcium load played a role in producing renal function impairment induced by diatrizoate meglumine; amlodipine protected the renal tissue from nephrotoxicity induced by diatrizoate.
评估高渗和低渗造影剂（HOCM、LOCM）对Sprague-Dawley大鼠肾脏的肾毒性。研究氨氯地平的保护作用。
40只雌雄大鼠随机分为5组（每组n = 8），除对照组外，所有大鼠均给予甘油以诱导肾衰竭。
注射泛影葡胺的大鼠血尿素氮（BUN）和血清肌酐（SCr）升高；肾组织中磷脂酶A2（PLA2）、脂质过氧化物（LPO）和钙水平也升高。在PLA2、LPO和钙的肾水平以及BUN和SCr水平方面，LOCM（碘海醇）组动物与甘油对照组之间无显著差异。LOCM组动物的组织学变化比HOCM组动物更轻。在氨氯地平预处理组中，未发现BUN或SCr水平升高，且PLA2、LPO和钙的肾含量显著低于HOCM组；泛影葡胺诱导的肾损伤得到缓解。
高渗造影剂泛影葡胺对大鼠肾脏的毒性比低渗造影剂碘海醇更大；PLA2、LPO和钙负荷在泛影葡胺诱导的肾功能损害中起作用；氨氯地平可保护肾组织免受泛影葡胺诱导的肾毒性。
