Riggio M P, Lennon A, Wray D
Infection Research Group, University of Glasgow Dental School, UK.
J Oral Pathol Med. 2000 Nov;29(10):507-13. doi: 10.1034/j.1600-0714.2000.291005.x.
Helicobacter pylori is recognised as being an aetiological agent of chronic active gastritis and peptic ulcer disease and has been associated with an increased risk of gastric cancer. The natural reservoir for H. pylori is unknown, although the oral cavity has been the focus of much attention in this respect. Given the histological similarities between gastric and oral ulceration, it seemed prudent to investigate a possible association between H. pylori and recurrent aphthous stomatitis (RAS). In this study, the potential involvement of H. pylori in the aetiology of RAS was investigated using the polymerase chain reaction (PCR). Biopsies from 28 RAS patients were analysed, in addition to 20 oral lichen planus (OLP) and 13 normal biopsies that were used as controls. Genomic DNA was extracted from biopsies, and confirmation of successful extraction of PCR-amplifiable DNA was achieved by carrying out PCR on each DNA sample with nested primers specific for the human beta-haemoglobin gene. PCR identification of H. pylori was carried out using a primer pair specific for the H. pylori 16S ribosomal RNA (rRNA) gene. Two rounds of PCR were carried out to amplify a 295-bp product, and the identity of amplified products was confirmed by DNA sequencing. H. pylori DNA was detected in 3 of 28 (11%) RAS samples but not in any of 20 OLP and 13 normal samples. These results do not support a definitive aetiological role for H. pylori in RAS, although the possibility that H. pylori may be involved in a small proportion of RAS cases cannot be excluded.
幽门螺杆菌被认为是慢性活动性胃炎和消化性溃疡病的病原体，并且与胃癌风险增加有关。幽门螺杆菌的自然宿主尚不清楚，尽管口腔在这方面备受关注。鉴于胃溃疡和口腔溃疡在组织学上的相似性，研究幽门螺杆菌与复发性阿弗他口炎（RAS）之间可能存在的关联似乎是明智的。在本研究中，使用聚合酶链反应（PCR）研究了幽门螺杆菌在RAS病因学中的潜在作用。除了将20例口腔扁平苔藓（OLP）和13例正常活检组织用作对照外，还分析了28例RAS患者的活检组织。从活检组织中提取基因组DNA，并通过使用针对人β-珠蛋白基因的巢式引物对每个DNA样本进行PCR来确认成功提取了可用于PCR扩增的DNA。使用针对幽门螺杆菌16S核糖体RNA（rRNA）基因的引物对进行幽门螺杆菌的PCR鉴定。进行两轮PCR以扩增出一个295bp的产物，并通过DNA测序确认扩增产物的身份。在28例RAS样本中的3例（11%）中检测到幽门螺杆菌DNA，但在20例OLP样本和13例正常样本中均未检测到。这些结果不支持幽门螺杆菌在RAS中具有明确的病因学作用，尽管不能排除幽门螺杆菌可能参与一小部分RAS病例的可能性。
