Fineschi S, Taurchini D, Villani F, Vendramin G G
Istituto per l'Agroselvicoltura, Consiglio Nazionale delle Ricerche, via Marconi 2, I-05010 Porano, Italy.
Mol Ecol. 2000 Oct;9(10):1495-503. doi: 10.1046/j.1365-294x.2000.01029.x.
The distribution of haplotypic diversity of 38 European chestnut (Castanea sativa Mill.) populations was investigated by PCR/RFLP analysis of regions of the chloroplast and mitochondrial genomes in order to shed light on the history of this heavily managed species. The rapid expansion of chestnut starting from 3000 years ago is strongly related to human activities such as agricultural practice. This demonstrates the importance of human impact, which lasted some thousands of years, on the present-day distribution of the species. No polymorphism was detected for the single mitochondrial analysed region, while a total of 11 different chloroplast (cp) haplotypes were scored. The distribution of the cpDNA haplotypes revealed low geographical structure of the genetic diversity. The value of population subdivision, as measured by GSTc, is strikingly lower than in the other species of the family Fagaceae investigated. The actual distribution of haplotypic diversity may be explained by the strong human impact on this species, particularly during the Roman civilization of the continent, and to the long period of cultivation experienced during the last thousand years.
通过对叶绿体和线粒体基因组区域进行PCR/RFLP分析，研究了38个欧洲栗（Castanea sativa Mill.）种群的单倍型多样性分布，以便深入了解这个受到严格管理的物种的历史。从3000年前开始，栗树的迅速扩张与农业实践等人类活动密切相关。这表明了持续数千年的人类影响对该物种现今分布的重要性。在所分析的单个线粒体区域未检测到多态性，而总共记录了11种不同的叶绿体（cp）单倍型。cpDNA单倍型的分布显示出遗传多样性的地理结构较低。通过GSTc测量的种群细分值明显低于所研究的壳斗科其他物种。单倍型多样性的实际分布可能是由于人类对该物种的强烈影响，特别是在欧洲大陆的罗马文明时期，以及过去一千年来经历的长期栽培。
