Paris-Palacios S, Biagianti-Risbourg S, Fouley A, Vernet G
Laboratoire d'Eco-Toxicologie, Faculté des Sciences, Institut International de Recherche sur les Ions Métalliques, Université de Reims Champagne-Ardenne, France.
Comp Biochem Physiol C Toxicol Pharmacol. 2000 Jun;126(2):113-22. doi: 10.1016/s0742-8413(00)00103-1.
Metallothioneins (MTs) have important roles in the homeostasis of essential metals and in the detoxication of heavy metals. They also represent a potential indicator of aquatic contamination by metals. Routine methods are needed for MTs quantification in ecotoxicological studies. This paper investigates the possibility to use the spectrofluorescent properties of Cu-MTs for MTs quantification. Cu displacement of metals coordinated to MTs and spectrofluorimetric determination of the obtained Cu-MTs was tested with commercial MTs and Cu2+-induced MTs in roach liver (Rutilus rutilus). Results of this original and simple spectrofluorimetric quantification of MTs presented a good correlation with data obtained with SH quantification, but not with metal summation evaluation of MTs (analysis of Zn, Cu and Cd coordinated to MTs). The three methods showed an clear induction of MTs in roach liver after 7 days of Cu2+ exposure. After 14 days of contamination, a reduction of hepatic MTs content was observable and not correlated to liver recovery. Results show that this low cost spectrofluorimetric method is useful to quantify MTs.
金属硫蛋白（MTs）在必需金属的体内平衡以及重金属解毒过程中发挥着重要作用。它们也是金属对水生环境造成污染的一个潜在指标。在生态毒理学研究中，需要常规方法来定量测定MTs。本文探讨了利用铜结合型金属硫蛋白（Cu-MTs）的荧光光谱特性来定量测定MTs的可能性。在蟑螂肝脏（Rutilus rutilus）中，用市售MTs和Cu²⁺诱导产生的MTs测试了与MTs配位的金属的铜置换以及对所得Cu-MTs的荧光分光光度测定。这种原始且简单的MTs荧光光谱定量结果与通过巯基（SH）定量获得的数据具有良好的相关性，但与MTs的金属总量评估（分析与MTs配位的锌、铜和镉）不相关。这三种方法均显示，在Cu²⁺暴露7天后，蟑螂肝脏中的MTs明显诱导生成。污染14天后，可观察到肝脏MTs含量降低，且与肝脏恢复情况无关。结果表明，这种低成本的荧光光谱法可用于定量测定MTs。
