Ingram G C, Doyle S, Carpenter R, Schultz E A, Simon R, Coen E S
John Innes Centre, Norwich, UK.
EMBO J. 1997 Nov 3;16(21):6521-34. doi: 10.1093/emboj/16.21.6521.
The fimbriata (fim) gene of Antirrhinum affects both the identity and arrangement of organs within the flower, and encodes a protein with an F-box motif. We show that FIM associates with a family of proteins, termed FAPs (FIM-associated proteins), that are closely related to human and yeast Skp1 proteins. These proteins form complexes with F-box-containing partners to promote protein degradation and cell cycle progression. The fap genes are expressed in inflorescence and floral meristems in a pattern that incorporates the domain of fim expression, supporting an in vivo role for a FIM-FAP complex. Analysis of a series of novel fim alleles shows that fim plays a key role in the activation of organ identity genes. In addition, fim acts in the regions between floral organs to specify the correct positioning and maintenance of morphological boundaries. Taking these results together, we propose that FIM-FAP complexes affect both gene expression and cell division, perhaps by promoting selective degradation of regulatory proteins. This may provide a mechanism by which morphological boundaries can be aligned with domains of gene expression during floral development.
金鱼草的fimbriata（fim）基因影响花内器官的特征和排列，并编码一种具有F-box基序的蛋白质。我们发现FIM与一类称为FAPs（FIM相关蛋白）的蛋白质相关联，这些蛋白质与人类和酵母的Skp1蛋白密切相关。这些蛋白质与含F-box的伴侣形成复合物，以促进蛋白质降解和细胞周期进程。fap基因在花序和花分生组织中以包含fim表达域的模式表达，支持FIM-FAP复合物在体内的作用。对一系列新的fim等位基因的分析表明，fim在器官特征基因的激活中起关键作用。此外，fim在花器官之间的区域起作用，以确定形态边界的正确定位和维持。综合这些结果，我们提出FIM-FAP复合物可能通过促进调节蛋白的选择性降解来影响基因表达和细胞分裂。这可能提供了一种机制，通过该机制在花发育过程中形态边界可以与基因表达域对齐。
