Garrido-Ramirez E R, Sudarshana M R, Lucas W J, Gilbertson R L
Department of Plant Pathology, University of California, Davis 95616, USA.
Mol Plant Microbe Interact. 2000 Nov;13(11):1184-94. doi: 10.1094/MPMI.2000.13.11.1184.
The capacities of the begomoviruses Bean dwarf mosaic virus (BDMV) and Bean golden yellow mosaic virus (BGYMV) to differeBean dwarf mosaic viru certain common bean (Phaseolus vulgaris) cultivars were used to identify viral determinants of the hypersensitive response (HR) and avirulence (avr) in BDMV. A series of hybrid DNA-B components, containing BDMV and BGYMV sequences, was constructed and coinoculated with BDMV DNA-A (BDMV-A) or BDMVA-green florescent protein into seedlings of cv. Topcrop (susceptible to BDMV and BGYMV) and the BDMV-resistant cvs. Othello and Black Turtle Soup T-39 (BTS). The BDMV avr determinant, in bean hypocotyl tissue, was mapped to the BDMV BV1 open reading frame and, most likely, to the BV1 protein. The BV1 also was identified as the determinant of the HR in Othello. However, the HR was not required for resistance in Othello nor was it associated with BDMV resistance in BTS. BDMV BV1, a nuclear shuttle protein that mediates viral DNA export from the nucleus, represents a new class of viral avr determinant. These results are discussed in terms of the relationship between the HR and resistance.
利用菜豆矮化花叶病毒（BDMV）和菜豆金黄花叶病毒（BGYMV）对某些普通菜豆（Phaseolus vulgaris）品种的侵染能力，来鉴定BDMV中过敏反应（HR）和无毒（avr）的病毒决定因素。构建了一系列包含BDMV和BGYMV序列的杂种DNA-B组分，并将其与BDMV DNA-A（BDMV-A）或BDMV-A-绿色荧光蛋白共同接种到Topcrop品种（对BDMV和BGYMV敏感）以及抗BDMV的Othello和黑龟汤T-39（BTS）品种的幼苗中。在菜豆下胚轴组织中，BDMV的avr决定因素被定位到BDMV的BV1开放阅读框，很可能是BV1蛋白。BV1也被确定为Othello中HR的决定因素。然而，Othello的抗性不需要HR，且HR与BTS对BDMV的抗性无关。BDMV BV1是一种介导病毒DNA从细胞核输出的核穿梭蛋白，代表了一类新的病毒avr决定因素。本文根据HR与抗性之间的关系对这些结果进行了讨论。
