酶联免疫吸附试验和血凝抑制试验检测鸵鸟（Struthio camelus）新城疫病毒抗体的比较。

Comparison of enzyme-linked immunosorbent assay and haemagglutination inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus).

机构信息

Department of Biochemistry, Faculty of Science, University of Zimbabwe, Harare, Zimbabwe.

出版信息

Avian Pathol. 1997;26(2):357-63. doi: 10.1080/03079459708419218.

DOI:10.1080/03079459708419218

Abstract

Reactivity of ostrich sera to Newcastle disease virus (NDV) by enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI) test were compared. Ten-month old ostriches seronegative by both tests were vaccinated with an oil-based NDV vaccine on days 0 and 11. Significant levels of reactive antibodies were first detected on day 11 by ELISA (sample/positive ratio > 0.2 in 11/20 birds; 55%) and HI (titre > 1/8 in 10/20 birds; 50%). At the end of the experiment (day 37) all birds had significant antibody levels by ELISA, but only 16/20 (80%) by HI test. There was a sigmoidal relationship (r= 0.62, 3rd degree polynomial) between antibody levels detected by ELISA and by HI test. Antibodies reactive with NDV in naturally exposed ostriches from Zimbabwe and Botswana were also detected by ELISA (112/165; 68%) and HI (85/165; 52%).

摘要

比较了酶联免疫吸附试验（ELISA）和血凝抑制（HI）试验检测鸵鸟血清中新城疫病毒（NDV）的反应性。通过两种检测方法均为阴性的 10 月龄鸵鸟在第 0 天和第 11 天接种油基 NDV 疫苗。ELISA 检测到第 11 天（20 只中有 11 只的样本/阳性比值 > 0.2，即 55%）和 HI 检测到第 11 天（20 只中有 10 只的 HI 效价 > 1/8，即 50%）首次出现明显的反应性抗体水平。在实验结束时（第 37 天），所有鸟类的 ELISA 抗体水平均显著升高，但仅 16/20 只（80%）的 HI 检测呈阳性。ELISA 和 HI 检测到的抗体水平之间呈 S 型关系（r=0.62，三次多项式）。在津巴布韦和博茨瓦纳自然暴露的鸵鸟中也检测到与 NDV 反应的抗体，ELISA（165 只中有 112 只，即 68%）和 HI（165 只中有 85 只，即 52%）。