Daniel T M, Ellner J J, Todd L S, McCoy D W, Payne V D, Anderson P A, Bhe F T
Infect Immun. 1979 Apr;24(1):77-82. doi: 10.1128/iai.24.1.77-82.1979.
The immunobiology and mycobacterial species distribution of immunoabsorbent affinity chromatography-purified Mycobacterium tuberculosis antigen 5 have been studied. In delayed hypersensitivity skin tests, antigen 5 was nearly equipotent with tuberculin-purified protein derivative in sensitized guinea pigs. In vitro, antigen 5 was capable of stimulating the production of migration inhibitory factor by cultured lymphocytes from sensitized guinea pigs and humans. Antigen 5 stimulated thymidine incorporation by cultured guinea pig lymphocytes but did not stimulate thymidine incorporation by cultured human lymphocytes. Although erythrocytes were readily sensitized with antigen 5 for passive hemagglutination, their use did not offer any advantage over previous hemagglutination techniques for the serodiagnosis or evaluation of patients with tuberculosis. By immunoelectrophoresis and immunodiffusion, antigen 5 was readily identified in culture filtrates of 10 strains of M. tuberculosis and M. bovis but not in those of 30 strains of 12 other myobacterial species.
对免疫吸附亲和层析纯化的结核分枝杆菌抗原5的免疫生物学和分枝杆菌种类分布进行了研究。在迟发型超敏皮肤试验中，抗原5在致敏豚鼠中与结核菌素纯化蛋白衍生物的效力几乎相当。在体外，抗原5能够刺激来自致敏豚鼠和人类的培养淋巴细胞产生迁移抑制因子。抗原5刺激培养的豚鼠淋巴细胞掺入胸腺嘧啶核苷，但不刺激培养的人类淋巴细胞掺入胸腺嘧啶核苷。虽然红细胞很容易用抗原5致敏用于被动血凝试验，但与先前用于结核病患者血清诊断或评估的血凝技术相比，其使用并没有任何优势。通过免疫电泳和免疫扩散，在10株结核分枝杆菌和牛分枝杆菌的培养滤液中很容易鉴定出抗原5，但在其他12种分枝杆菌的30株菌株的培养滤液中未鉴定出。
