铜绿假单胞菌对兔多形核白细胞吞噬和杀伤活性的抑制作用：多形核白细胞功能抑制剂的纯化与特性分析
Inhibitory effect of Pseudomonas aeruginosa on the phagacytic and killing activities of rabbit polymorphonuclear leukocytes: purification and characterization of an inhibitor of polymorphonuclear leukocyte function.
作者信息
Nonoyama S, Kojo H, Mine Y, Nishida M, Goto S, Kuwahara S
出版信息
Infect Immun. 1979 May;24(2):394-8. doi: 10.1128/iai.24.2.394-398.1979.
Abstract
A clinically isolated strain of polymorphonuclear leukocyte (PMN)-resistant Pseudomonas was found to produce an extracellular substance (PMN inhibitor) that inhibits the phagocytic and killing activities of PMN. The PMN inhibitor was purified from culture filtrates by precipitation with (NH4)2SO4 and chromatography on phosphocellulose, diethylaminoethyl-cellulose, and Sephadex G-100. The preparation was homogenous as judged by disc gel electrophoresis. The purified PMN inhibitor appeared to be a protein with a molecular weight of approximately 65,000 that was inactivated by heating and by exposure to a proteolytic enzyme.
摘要
发现一株临床上分离出的对多形核白细胞（PMN）具有抗性的假单胞菌能产生一种细胞外物质（PMN抑制剂），该物质可抑制PMN的吞噬和杀伤活性。通过用硫酸铵沉淀以及在磷酸纤维素、二乙氨基乙基纤维素和葡聚糖G - 100上进行色谱分离，从培养滤液中纯化出了PMN抑制剂。根据圆盘凝胶电泳判断，该制剂是纯的。纯化后的PMN抑制剂似乎是一种分子量约为65000的蛋白质，加热和暴露于蛋白水解酶会使其失活。
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