Whitby J E, Heaton P R, Black E M, Wooldridge M, McElhinney L M, Johnstone P
The Wellcome Trust, London.
Vet Rec. 2000 Sep 30;147(14):385-8. doi: 10.1136/vr.147.14.385.
On May 30, 1996, a sick Daubenton's bat (Myotis daubentonii) was recovered from the cellar of a public house in Newhaven, East Sussex. Its condition deteriorated rapidly, and it was euthanased and examined. Positive results, establishing the presence of a rabies or rabies-related virus in its brain, were obtained from the fluorescent antibody test, the rabies tissue culture isolation test, and a hemi-nested reverse-transcription PCR. The complete sequence of the nucleoprotein gene was determined and a phylogenetic analysis, based on the 470 nucleotide bases of the amino terminus of the nucleoprotein, established the genotype of the virus as European bat lyssavirus 2. Bat rabies had not previously been recorded in the UK but does occur in mainland Europe. A study of the back-trajectories of the wind on May 29 and 30, established that the infected bat possibly came from near the Franco-Swiss border.
1996年5月30日,一只生病的道氏鼠耳蝠(Myotis daubentonii)在东萨塞克斯郡纽黑文一家酒馆的地窖中被发现。其病情迅速恶化,随后被实施安乐死并进行检测。通过荧光抗体检测、狂犬病组织培养分离试验以及半巢式逆转录聚合酶链反应,均得到阳性结果,确定其大脑中存在狂犬病病毒或狂犬病相关病毒。测定了核蛋白基因的完整序列,并基于核蛋白氨基末端的470个核苷酸碱基进行了系统发育分析,确定该病毒的基因型为欧洲蝙蝠狂犬病病毒2型。此前英国未曾有过蝙蝠狂犬病的记录,但在欧洲大陆确实存在。对5月29日和30日风向轨迹的研究表明,这只受感染的蝙蝠可能来自法国-瑞士边境附近。