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利用荧光抗体技术对放线菌进行血清学鉴定。

Serological identification of Actinomyces using fluorescent antibody techniques.

作者信息

Gerencser M A, Slack J M

出版信息

J Dent Res. 1976 Jan;55:A184-91. doi: 10.1177/002203457605500110011.

DOI:10.1177/002203457605500110011
PMID:1107378
Abstract

Species-specific FITC conjugated antiserum can be prepared for each of the five species of Actinomyces and for Arachnia propionica. These serums can be used for rapid and specific identification of pure or mixed cultures of the bacteria and for identification of organisms seen in direct smears of clinical material or in tissue sections. Two serotypes each of A bovis, A odontolyticus, A israelii, A viscosus, and A propionica have been established, and A naeslundii has been tentatively divided into four serotypes.

摘要

可以为放线菌属的五个菌种以及丙酸蛛网菌分别制备种特异性异硫氰酸荧光素(FITC)偶联抗血清。这些抗血清可用于快速、特异性地鉴定细菌的纯培养物或混合培养物,以及鉴定临床材料直接涂片或组织切片中所见的微生物。已确定牛放线菌、溶齿放线菌、衣氏放线菌、黏性放线菌和丙酸蛛网菌各有两种血清型,而内氏放线菌已初步分为四种血清型。

相似文献

1
Serological identification of Actinomyces using fluorescent antibody techniques.利用荧光抗体技术对放线菌进行血清学鉴定。
J Dent Res. 1976 Jan;55:A184-91. doi: 10.1177/002203457605500110011.
2
Immunocytochemical demonstration of Actinomyces species and Arachnia propionica in periapical infections.放线菌属及丙酸蛛网菌在根尖感染中的免疫细胞化学证明
J Oral Pathol. 1985 May;14(5):405-13. doi: 10.1111/j.1600-0714.1985.tb00512.x.
3
Antigenic relationships among oral Actinomyces isolates, Actinomyces naeslundii genospecies 1 and 2, Actinomyces howellii, Actinomyces denticolens, and Actinomyces slackii.口腔放线菌分离株、内氏放线菌基因种1和2、霍氏放线菌、齿龈放线菌和斯氏放线菌之间的抗原关系。
J Dent Res. 1993 Oct;72(10):1374-85. doi: 10.1177/00220345930720100601.
4
Immune labeling of certain strains of Actinomyces naeslundii and Actinomyces viscosus by fluorescence and electron microscopy.通过荧光显微镜和电子显微镜对某些内氏放线菌和黏性放线菌菌株进行免疫标记。
Infect Immun. 1979 Sep;25(3):1016-28. doi: 10.1128/iai.25.3.1016-1028.1979.
5
Distribution of antigenic determinants between Actinomyces viscosus and Actinomyces naeslundii.粘性放线菌与内氏放线菌之间抗原决定簇的分布
J Dent Res. 1988 Jan;67(1):15-20. doi: 10.1177/00220345880670010101.
6
Identification of Actinomyces israelii and Actinomyces naeslundii by fluorescent-antibody and agar-gel diffusion techniques.用荧光抗体和琼脂凝胶扩散技术鉴定以色列放线菌和内氏放线菌。
J Bacteriol. 1967 Nov;94(5):1287-95. doi: 10.1128/jb.94.5.1287-1295.1967.
7
Determination and analysis of Actinomyces israelii serotypes by fluorescent-antibody procedures.采用荧光抗体法对以色列放线菌血清型进行鉴定与分析。
J Bacteriol. 1969 Feb;97(2):581-8. doi: 10.1128/jb.97.2.581-588.1969.
8
Direct detection of Actinomyces spp. from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique.在中国人群中直接检测感染根管内的放线菌属:一项基于聚合酶链反应的寡核苷酸 - DNA杂交技术研究
J Dent. 2003 Nov;31(8):559-68. doi: 10.1016/s0300-5712(03)00112-x.
9
Demonstration of Actinomyces and Arachnia species in cervicovaginal smears by direct staining with species-specific fluorescent-antibody conjugate.通过用种特异性荧光抗体结合物直接染色在宫颈阴道涂片中显示放线菌属和蛛形纲菌种。
J Clin Microbiol. 1981 Jan;13(1):15-21. doi: 10.1128/jcm.13.1.15-21.1981.
10
Actinomyces of infected dental root canals.
Oral Surg Oral Med Oral Pathol. 1981 Jun;51(6):643-8. doi: 10.1016/s0030-4220(81)80016-3.

引用本文的文献

1
Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963.内氏放线菌的修订描述以及口腔放线菌新种、约翰逊放线菌新种的描述,这两种菌之前被鉴定为内氏放线菌基因种1、2和WVA 963。
Int J Syst Evol Microbiol. 2009 Mar;59(Pt 3):509-16. doi: 10.1099/ijs.0.000950-0.
2
Identification and preliminary characterization of a major heat-stable surface antigen of Actinomyces israelii by two-dimensional (crossed) immunoelectrophoresis.通过二维(交叉)免疫电泳对以色列放线菌主要热稳定表面抗原进行鉴定和初步表征。
Infect Immun. 1983 Jul;41(1):11-8. doi: 10.1128/iai.41.1.11-18.1983.
3
Chemical and immunological comparison of surface fibrils of strains representing six taxonomic groups of Actinomyces viscosus and Actinomyces naeslundii.
黏性放线菌和内氏放线菌六个分类群代表菌株表面纤丝的化学和免疫学比较。
Infect Immun. 1983 Mar;39(3):1325-33. doi: 10.1128/iai.39.3.1325-1333.1983.
4
Assignment of Actinomyces viscosus and Actinomyces naeslundii strains to numerical taxonomy clusters by immunofluorescence based on antifibril antisera.基于抗纤丝抗血清,通过免疫荧光将黏性放线菌和内氏放线菌菌株归类到数值分类学聚类中。
J Clin Microbiol. 1985 May;21(5):850-3. doi: 10.1128/jcm.21.5.850-853.1985.
5
Conservation of an Actinomyces viscosus T14V type 1 fimbrial subunit homolog among divergent groups of Actinomyces spp.粘性放线菌T14V 1型菌毛亚基同源物在不同放线菌属菌群中的保守性
Infect Immun. 1992 Mar;60(3):1047-54. doi: 10.1128/iai.60.3.1047-1054.1992.
6
Establishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity.粘性放线菌和内氏放线菌在人类口腔中的定植与分布。
Infect Immun. 1976 Nov;14(5):1119-24. doi: 10.1128/iai.14.5.1119-1124.1976.
7
Rapid method for identification and enumeration of oral Actinomyces.口腔放线菌鉴定与计数的快速方法
Infect Immun. 1978 Sep;21(3):786-91. doi: 10.1128/iai.21.3.786-791.1978.
8
Immune labeling of certain strains of Actinomyces naeslundii and Actinomyces viscosus by fluorescence and electron microscopy.通过荧光显微镜和电子显微镜对某些内氏放线菌和黏性放线菌菌株进行免疫标记。
Infect Immun. 1979 Sep;25(3):1016-28. doi: 10.1128/iai.25.3.1016-1028.1979.