Montanaro L, Arciola C R, Cenni E, Ciapetti G, Savioli F, Filippini F, Barsanti L A
Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
Biomaterials. 2001 Jan;22(1):59-66. doi: 10.1016/s0142-9612(00)00163-0.
The biocompatibility of two cyanoacrylate surgical glues (Glubran and Glubran 2), supplied by General Enterprise Marketing, Viareggio, Lucca, Italy, was tested through cytotoxicity and blood compatibility tests and the evaluation of antimicrobial activity. Cytotoxicity and blood compatibility tests were performed on the polymerized glues. Using the neutral red uptake test, the extracts from Glubran and Glubran 2 after polymerization were non-toxic to L929 cells only when diluted 1: 10 with culture medium. Glubran and Glubran 2 induced a significant decrease of activated partial thromboplastin time (APTT), which is favourable with regard to the desired haemostasis. The APTT shortening determines a haemostatic effect and therefore contribute to the tissue adhesion induced by the glues. Otherwise, no significant variation of prothrombin activity, fibrinogen, platelet number, total and differential leukocyte count was induced by the glues, which, in addition, did not show haemolytic effect. There was no difference between Glubran and Glubran 2 regarding haemocompatibility. The antimicrobial ability of the unpolymerized glues was tested onto Bacillus subtilis var. niger for 3 weeks: neither Glubran nor Glubran 2 were found effective in this respect. In conclusion, we can assume that cytotoxicity was severe with the undiluted glues, but was acceptable when glues were diluted. On the contrary, blood compatibility was acceptable for the intended use of the glues. No difference was found between Glubran and Glubran 2 after polymerization.
由意大利卢卡维亚雷焦的通用企业营销公司提供的两种氰基丙烯酸酯手术胶水（Glubran和Glubran 2）的生物相容性，通过细胞毒性和血液相容性测试以及抗菌活性评估进行了检测。对聚合后的胶水进行了细胞毒性和血液相容性测试。使用中性红摄取试验，聚合后的Glubran和Glubran 2提取物仅在以培养基1:10稀释时对L929细胞无毒。Glubran和Glubran 2可使活化部分凝血活酶时间（APTT）显著缩短，这对于预期的止血是有利的。APTT缩短决定了止血效果，因此有助于胶水诱导的组织粘连。此外，胶水未引起凝血酶原活性、纤维蛋白原、血小板数量、白细胞总数及分类计数的显著变化，且未表现出溶血作用。在血液相容性方面，Glubran和Glubran 2之间没有差异。对未聚合的胶水在黑曲霉上进行了3周的抗菌能力测试：在这方面，未发现Glubran和Glubran 2有效。总之，我们可以认为未稀释的胶水细胞毒性严重，但稀释后是可接受的。相反，胶水在预期用途中的血液相容性是可接受的。聚合后，Glubran和Glubran 2之间未发现差异。
