Czinner E, Hagymási K, Blázovics A, Kéry A, Szoke E, Lemberkovics E
Department of Pharmacognosy, Faculty of Pharmacy, Semmelweis University, Ulloi út 26, 1085, Budapest, Hungary.
J Ethnopharmacol. 2000 Dec;73(3):437-43. doi: 10.1016/s0378-8741(00)00304-4.
The choleretic, hepatoprotective and detoxifying activities of the inflorescence of Helichrysum arenarium (L.) Moench (everlasting, immortelle: Asteraceae-Helichrysi flos syn. Stoechados flos) have been known for a long time from herbal medicine in Hungary. Antioxidant properties of its main phenolics, flavonoids, are supposed to be responsible for these effects. The aim of this study is to verify the antioxidant properties of the lyophilized water extracts from inflorescences, and to define the total polyphenol and flavonoid contents in Helichrysi flos water extracts as well as in lyophilized water extracts. The hydrogen-donating ability and the reducing power property of the lyophilizates were determined spectrophotometrically; their OH&z.rad; scavenging activity was measured, in the H(2)O(2)/OH&z.rad;-luminol-microperoxidase system, by a chemiluminometric method. Results were compared with the activity of the flavonoid silibinin, the main agent of the well-known milk thistle (Silybum marianum L.).
匈牙利草药学早就知晓蜡菊（Helichrysum arenarium (L.) Moench，永生菊、不凋花：菊科-蜡菊属，同物异名Stoechados flos）花序的利胆、保肝和解毒活性。其主要酚类物质、黄酮类化合物的抗氧化特性被认为是这些作用的原因。本研究的目的是验证花序冻干水提取物的抗氧化特性，并确定不凋花水提取物以及冻干水提取物中的总多酚和黄酮含量。通过分光光度法测定冻干物的供氢能力和还原能力；在H₂O₂/OH˙-鲁米诺-微过氧化物酶体系中，采用化学发光法测定其OH˙清除活性。将结果与著名的水飞蓟（Silybum marianum L.）的主要成分黄酮类化合物水飞蓟宾的活性进行比较。
