Hazbón M H, del Socorro Orozco M, Labrada L A, Tovar R, Weigle K A, Wanger A
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Cali, Colombia.
J Clin Microbiol. 2000 Dec;38(12):4599-603. doi: 10.1128/JCM.38.12.4599-4603.2000.
To prescribe effective treatment schemes for patients with tuberculosis, more-efficient susceptibility testing techniques for Mycobacterium tuberculosis are needed, especially in regions with multidrug resistance. Etest (AB BIODISK, Solna, Sweden) is a simple technique that provides quantitative drug susceptibility results for M. tuberculosis in 5 to 10 days from a culture grown at low cost. The performance of Etest was compared to that of the reference proportion method, using 95 M. tuberculosis clinical isolates of which 42.1% (40 of 95) were resistant to at least one antibiotic by the reference method. Overall agreement between Etest and the reference method was 98.9% (94 of 95) for detection of multidrug resistance; for resistance to individual drugs, agreement was 97.9% (93 of 95) for rifampin, 96.0% (92 of 95) for ethambutol, 94.7% (90 of 95) for isoniazid, and 85.3% (81 of 95) for streptomycin. This study supports the utility of Etest for timely detection of drug resistance in M. tuberculosis and for use in tuberculosis control programs.
为了给结核病患者制定有效的治疗方案，需要更高效的结核分枝杆菌药敏试验技术，尤其是在多重耐药地区。Etest（AB BIODISK，瑞典索尔纳）是一种简单的技术，能在5至10天内从低成本培养物中为结核分枝杆菌提供定量药敏结果。使用95株结核分枝杆菌临床分离株将Etest的性能与参考比例法进行比较，其中42.1%（95株中的40株）通过参考方法对至少一种抗生素耐药。Etest与参考方法在检测多重耐药方面的总体一致性为98.9%（95株中的94株）；对于个别药物的耐药性，利福平的一致性为97.9%（95株中的93株），乙胺丁醇为96.0%（95株中的92株），异烟肼为94.7%（95株中的90株），链霉素为85.3%（95株中的81株）。本研究支持Etest在及时检测结核分枝杆菌耐药性以及用于结核病控制项目方面的实用性。
