铜绿假单胞菌随机扩增多态性DNA分型的评估
Evaluation of random amplified polymorphic DNA typing of Pseudomonas aeruginosa.
作者信息
Campbell M, Mahenthiralingam E, Speert D P
机构信息
Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, and Children's and Women's Health Centre of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada.
出版信息
J Clin Microbiol. 2000 Dec;38(12):4614-5. doi: 10.1128/JCM.38.12.4614-4615.2000.
Abstract
A method for distinguishing among Pseudomonas aeruginosa strains using random amplified polymorphic DNA (RAPD) typing was evaluated for reproducibility and discriminatory power. A total of 200 isolates, blinded in triplicate, were evaluated by RAPD. All 600 samples were typeable; 197 of 200 isolates gave identical results on all three occasions, and 131 distinct RAPD types were identified.
摘要
对一种使用随机扩增多态性DNA（RAPD）分型来区分铜绿假单胞菌菌株的方法进行了可重复性和鉴别力评估。总共200株分离菌被一式三份进行盲法检测，通过RAPD进行评估。所有600个样本均可分型；200株分离菌中的197株在所有三次检测中结果相同，共鉴定出131种不同的RAPD类型。
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