Geerlings S E, Brouwer E C, Van Kessel K C, Gaastra W, Stolk R P, Hoepelman A I
University Hospital Utrecht and Utrecht University, Utrecht, The Netherlands.
Eur J Clin Invest. 2000 Nov;30(11):995-1001. doi: 10.1046/j.1365-2362.2000.00745.x.
As women with diabetes mellitus (DM) have an increased prevalence of asymptomatic bacteriuria (ASB) and it is known that a correlation exists between the increased prevalence of genitourinary tract infection and impaired cytokine production in women infected with Human Immunodeficiency Virus (HIV), we studied urinary cytokine excretion in diabetic women and compared it with that of nondiabetic controls.
To evaluate the cytokine secretion capacity of women with DM, both whole blood and isolated monocytes of women with and without DM were stimulated in vitro with lipopolysaccharide (LPS).
Lower urinary interleukin-8 (IL-8) and interleukin-6 (IL-6) concentrations (P = 0.1 and P < 0.001, respectively) were found in diabetic women than in nondiabetic controls. A lower urinary leukocyte cell count correlated with lower urinary IL-8 and IL-6 concentrations (P < 0.05). Lower tumour necrosis factor-alpha (TNF-alpha) and IL-6, but comparable interleukin-10 (IL-10) concentrations were found in whole blood (P < 0.04) and isolated monocytes (P = 0.03) of women with DM type 1 compared to women without DM.
Diabetic women with ASB have lower urinary IL-6 concentrations than nondiabetic bacteriuric controls. In addition, monocytes of women with DM type 1 secrete lower pro-inflammatory cytokines after stimulation with LPS than monocytes of women without DM. This is not due to an inhibitory effect of the anti-inflammatory cytokine IL-10. This can have important consequences for both host defense, endothelial cell functioning and atherogenesis.
由于糖尿病（DM）女性无症状菌尿（ASB）的患病率增加，且已知感染人类免疫缺陷病毒（HIV）的女性中，泌尿生殖道感染患病率的增加与细胞因子产生受损之间存在相关性，我们研究了糖尿病女性的尿细胞因子排泄情况，并将其与非糖尿病对照组进行比较。
为评估糖尿病女性的细胞因子分泌能力，对患有和未患有DM的女性的全血和分离的单核细胞均用脂多糖（LPS）进行体外刺激。
发现糖尿病女性的尿白细胞介素-8（IL-8）和白细胞介素-6（IL-6）浓度低于非糖尿病对照组（分别为P = 0.1和P < 0.001）。较低的尿白细胞计数与较低的尿IL-8和IL-6浓度相关（P < 0.05）。与未患DM的女性相比，1型糖尿病女性的全血（P < 0.04）和分离的单核细胞（P = 0.03）中肿瘤坏死因子-α（TNF-α）和IL-6较低，但白细胞介素-10（IL-10）浓度相当。
患有ASB的糖尿病女性的尿IL-6浓度低于非糖尿病菌尿对照组。此外，1型糖尿病女性的单核细胞在用LPS刺激后分泌的促炎细胞因子低于未患DM的女性的单核细胞。这不是由于抗炎细胞因子IL-10的抑制作用。这可能对宿主防御、内皮细胞功能和动脉粥样硬化形成都有重要影响。
